Determination of medullasin levels for the diagnosis of multiple sclerosis.

Abstract

OBJECTIVES:

To obtain a simple and reliable clinical parameter for the diagnosis of multiple sclerosis among patients with neurological diseases.

PATIENTS AND METHODS:

Heparinized peripheral blood was obtained from patients with multiple sclerosis and those with non-inflammatory neurological diseases and healthy volunteers. A new enzyme immunoassay method determining medullasin levels in human granulocytes was developed by using mouse monoclonal antibody against medullasin.

RESULTS:

A newly developed enzyme immunoassay method for medullasin can detect as little as 1 ng/ml medullasin and results can be obtained within 2 h. Eighty-five out of 112 patients with multiple sclerosis (75.8%) showed positive results (above means of normals + 2 SD) in the medullasin test, while 15.4% (12/78) of patients with non-inflammatory neurological disease had positive results.

CONCLUSION:

This newly developed enzyme immunoassay method for medullasin is considered to be a useful paraclinical test for the diagnosis of multiple sclerosis.

• Authors: Y Aoki, T Saida, I Nakano, T Saito, K Ikeguchi, T Urabe, E Nishiguchi, H Suzuki, K Takahashi, H Katsuragi, Y Mizuno
• Journal: Acta neurologica Scandinavica (Acta Neurol Scand) Vol. 102 Issue 4 Pg. 218-21 (Oct 2000) ISSN: 0001-6314 [Print] Denmark
• PMID: 11071105 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Endopeptidases
• Serine Endopeptidases
• serine endopeptidase

Topics

• Adolescent
• Adult
• Antibodies, Monoclonal (immunology)
• Child
• Endopeptidases (blood, immunology)
• Female
• Granulocytes (enzymology)
• Humans
• Male
• Middle Aged
• Multiple Sclerosis (blood, diagnosis)
• Serine Endopeptidases