The Bilitec ambulatory
bile reflux monitor is advocated for the assessment of
bile reflux. However, it has only been validated by bench top (in vitro) studies. This in vivo study used controlled 'iatrogenic' episodes of
bile reflux to determine the ability of the Bilitec system to detect
bile acids in the oesophageal lumen. At least 10 ml of fluid was aspirated from the stomach of each of 32 patients, and analysed quantitatively for total
bile acids (TBA) and total
bilirubin (TB). An in vitro assessment of this fluid with the Bilitec probe was performed initially. The Bilitec probe was then sited in the distal oesophagus, and the gastric fluid was introduced into the mid-oesophagus, through a naso-oesophageal tube, as a series of 10-ml volumes of varying dilutions (1:8, 1:4, 1:2, undiluted) to simulate
gastro-oesophageal reflux events. Intraoesophageal
bilirubin absorbance was compared with TBA and TB for each dilution. Both TBA and TB values correlated strongly with
bilirubin absorbance in vitro (r = 0.83, p < 0.0001 and r = 0.82, p < 0.0001 respectively). However, this relationship was weaker in vivo (r = 0.64, p = 0.0001 and r = 0.68, p < 0.0001 respectively). Of the dilutions which contained potentially injurious concentrations of
bile acid (>1 mmol/L), 77% were associated with absorbance >0.14 (currently accepted absorbance threshold for oesophageal
bile reflux). Although in vitro sensitivity of the Bilitec probe to
bile acids was excellent, in vivo sensitivity was less reliable, and 23% of significant '
bile reflux' episodes did not exceed the currently recommended Bilitec absorbance threshold for
bile reflux. In contrast, false-positive results were unlikely. These findings suggest that the overall accuracy of the Bilitec system is probably sufficient for clinical use, although this method is not sufficiently reliable to be regarded as the gold standard for the evaluation of duodeno-oesophageal reflux.