Abstract |
The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H(t(His)):gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD(t) were determined. As antigens, purified soluble recombinant forms of the gH(t(His)):gL complex produced by insect cells and of gD(t) produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH(t(His)): gL-specific IgG. Using peripheral blood (PB) T cells, gH(t(His)):gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH(t(His)):gL contained high levels of interferon-gamma and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.
|
Authors | Douwe F Westra, Georges M G M Verjans, Albert D M E Osterhaus, Adriaan van Kooij, Gjalt W Welling, Albert Jan Scheffer, T Hauw The, Sytske Welling-Wester |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 81
Issue Pt 8
Pg. 2011-2015
(Aug 2000)
ISSN: 0022-1317 [Print] England |
PMID | 10900040
(Publication Type: Journal Article)
|
Chemical References |
- Antibodies, Viral
- Viral Envelope Proteins
- Interleukin-4
- Interferon-gamma
|
Topics |
- Animals
- Antibodies, Viral
(blood)
- Chlorocebus aethiops
- Herpesvirus 1, Human
(immunology)
- Humans
- Interferon-gamma
(biosynthesis)
- Interleukin-4
(biosynthesis)
- Lymphocyte Activation
- T-Lymphocytes
(immunology)
- Vero Cells
- Viral Envelope Proteins
(immunology)
|