Abstract | BACKGROUND: We previously reported that Fas antigen was strongly expressed on salivary duct epithelial cells and that some salivary infiltrating cells showed the Fas ligand in patients with severe sialoadenitis due to Sjögren's syndrome (SS). Apoptotic changes were observed in ductal epithelial cells and some infiltrating cells by DNA nick end labeling methods. These findings suggest that the Fas- Fas ligand system may play a role in the pathogenesis of sialoadenitis in SS. OBJECTIVE: METHODS: RESULTS: Unstimulated HSG cells constitutively expressed low levels of Fas antigen. IFN-gamma and TNF-alpha consistently upregulated constitutive levels of Fas. In contrast, IL-1 beta, IL-2, IL-4, and GM-CSF had no effect on Fas levels. HSG cells expressing Fas antigen in response to IFN-gamma or TNF-alpha were susceptible to apoptosis by anti-Fas antibody. CONCLUSION: Our findings suggest that IFN-gamma or TNF-alpha secreted by infiltrating lymphocytes induces ductal Fas expression and ductal apoptosis in sialoadenitis associated with SS.
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Authors | R Matsumura, K Umemiya, T Goto, T Nakazawa, K Ochiai, M Kagami, H Tomioka, E Tanabe, T Sugiyama, M Sueishi |
Journal | Clinical and experimental rheumatology
(Clin Exp Rheumatol)
2000 May-Jun
Vol. 18
Issue 3
Pg. 311-8
ISSN: 0392-856X [Print] Italy |
PMID | 10895367
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Immunoglobulin G
- Interleukin-1
- Interleukin-2
- Tumor Necrosis Factor-alpha
- fas Receptor
- Interleukin-4
- Interferon-gamma
- Granulocyte-Macrophage Colony-Stimulating Factor
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Topics |
- Antineoplastic Agents
(immunology, pharmacology)
- Apoptosis
(drug effects, immunology)
- Cell Line
- Epithelial Cells
(chemistry, cytology, drug effects)
- Fluorescent Antibody Technique
- Granulocyte-Macrophage Colony-Stimulating Factor
(pharmacology)
- Humans
- Immunoenzyme Techniques
- Immunoglobulin G
(pharmacology)
- Interferon-gamma
(immunology, pharmacology)
- Interleukin-1
(pharmacology)
- Interleukin-2
(pharmacology)
- Interleukin-4
(pharmacology)
- Lymphadenitis
(immunology, pathology)
- Neutralization Tests
- Salivary Ducts
(cytology)
- Sjogren's Syndrome
(immunology, pathology)
- Tumor Necrosis Factor-alpha
(pharmacology)
- fas Receptor
(analysis, biosynthesis)
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