Dominant negative myostatin produces hypertrophy without hyperplasia in muscle.

Abstract

Myostatin, a TGF-beta family member, is a negative regulator of muscle growth. Here, we generated transgenic mice that expressed myostatin mutated at its cleavage site under the control of a muscle specific promoter creating a dominant negative myostatin. These mice exhibited a significant (20-35%) increase in muscle mass that resulted from myofiber hypertrophy and not from myofiber hyperplasia. We also evaluated the role of myostatin in muscle degenerative states, such as muscular dystrophy, and found significant downregulation of myostatin. Thus, further inhibition of myostatin may permit increased muscle growth in muscle degenerative disorders.

• Authors: X Zhu, M Hadhazy, M Wehling, J G Tidball, E M McNally
• Journal: FEBS letters (FEBS Lett) Vol. 474 Issue 1 Pg. 71-5 (May 26 2000) ISSN: 0014-5793 [Print] England
• PMID: 10828454 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Mstn protein, mouse
• Myostatin
• RNA, Messenger
• Transforming Growth Factor beta

Topics

• Animals
• Blotting, Northern
• Gene Expression
• Gene Expression Regulation
• Hyperplasia
• Hypertrophy
• Mice
• Mice, Transgenic
• Muscle Fibers, Skeletal (pathology)
• Muscle, Skeletal (pathology)
• Muscular Dystrophy, Animal (metabolism)
• Mutagenesis
• Myostatin
• RNA, Messenger (metabolism)
• Transforming Growth Factor beta (genetics, physiology)