Utility of cytokeratin 7 for distinguishing chromophobe renal cell carcinoma from renal oncocytoma.

Abstract

OBJECTIVE:

Chromophobe renal cell carcinoma is a relatively uncommon variant of renal carcinoma described in 1985. The main differential diagnosis is renal oncocytoma. Hale's colloidal iron staining is a powerful adjunct to morphological interpretation but it is not specific and is sometimes difficult to interpret. We studied the immunohistochemical expression of cytokeratin 7 to determine its value in distinguishing chromophobe renal cell carcinoma from renal oncocytoma.

METHODS:

Immunostaining was performed on paraffin-embedded tumor tissue of 6 chromophobe renal cell carcinomas and 11 oncocytomas with an antibody to cytokeratin 7 (clone OV-TL 12/30, Dako, France) using a streptavidin-biotin method.

RESULTS:

All chromophobe renal cell carcinomas showed strong cytoplasmic staining with peripheral cell accentuation. In contrast, 8 of 11 oncocytomas were entirely negative and 3 showed only weak and focal staining in less than 5% of the tumor cells.

CONCLUSION:

Immunohistochemical staining for cytokeratin 7 may be useful for the differential diagnosis of renal oncocytomas and chromophobe renal cell carcinomas when Hale's colloidal iron staining is uncertain.

• Authors: X Leroy, D Moukassa, M C Copin, F Saint, E Mazeman, B Gosselin
• Journal: European urology (Eur Urol) Vol. 37 Issue 4 Pg. 484-7 (Apr 2000) ISSN: 0302-2838 [Print] Switzerland
• PMID: 10765081 (Publication Type: Clinical Trial, Comparative Study, Journal Article)

Chemical References

• KRT7 protein, human
• Keratin-7
• Keratins

Topics

• Adenocarcinoma (pathology, surgery)
• Adenoma, Oxyphilic (pathology, surgery)
• Adult
• Aged
• Aged, 80 and over
• Biopsy, Needle
• Carcinoma, Renal Cell (pathology, surgery)
• Diagnosis, Differential
• Female
• Humans
• Immunohistochemistry (methods)
• Keratin-7
• Keratins
• Kidney Neoplasms (pathology, surgery)
• Male
• Middle Aged
• Sensitivity and Specificity