Abstract | BACKGROUND: METHODS: In some experiments, mice were given OVA, liposomes or OVA- liposomes with or without CT intranasally three times, at 2-week intervals (weeks 0, 2 and 4). In other experiments, mice were given OVA- liposomes intranasally 2 days before or 1 and 3 weeks after CT-combined OVA (week 0), which was administered intranasally three times, at 2-week intervals (weeks 0, 2 and 4). Two weeks after the third administration of CT-combined OVA (week 0), nasal wash and serum IgA, IgG and IgE Ab responses were assayed. RESULTS: Pretreatment with OVA- liposomes suppressed IgE Ab responses to CT-combined OVA, with a significantly high production of both nasal IgA and serum IgG Abs. Moreover, treatment with OVA- liposomes 1 and 3 weeks after CT-combined OVA administration also suppressed IgE Ab responses. The suppression of anti-OVA IgE Ab production by OVA- liposomes was accompanied by a simultaneous enhancement of specific IgA and IgG ( IgG1, and especially IgG2a) Ab production. CONCLUSIONS: Postimmunization treatment with OVA- liposomes, as well as preimmunization treatment, suppressed specific IgE Ab responses in mice sensitized intranasally with CT-combined OVA. Allergens conjugated to liposomes may be appropriate for preventing the development of allergies to inhaled or dietary antigens in humans.
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Authors | T Yoshikawa, T Uchida, S Naito, A Horino, M Taneichi, H Kato, K Komuro, Y Nakano, M Mori, S Nishinohara, J Chiba, T Kurata, S Tamura |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 121
Issue 2
Pg. 108-15
(Feb 2000)
ISSN: 1018-2438 [Print] Switzerland |
PMID | 10705220
(Publication Type: Journal Article)
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Copyright | Copyright 2000 S. Karger AG, Basel |
Chemical References |
- Immunoglobulin A
- Immunoglobulin G
- Liposomes
- Immunoglobulin E
- Ovalbumin
- Cholera Toxin
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Topics |
- Administration, Intranasal
- Animals
- Antibody Specificity
- Cholera Toxin
(administration & dosage, immunology)
- Female
- Immunization
- Immunoglobulin A
(biosynthesis)
- Immunoglobulin E
(biosynthesis)
- Immunoglobulin G
(biosynthesis)
- Liposomes
(administration & dosage, immunology)
- Mice
- Mice, Inbred BALB C
- Nasal Mucosa
(immunology)
- Ovalbumin
(administration & dosage, immunology)
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