Abstract | PURPOSE: METHODS: RESULTS: The central and peripheral cellular components of normal sebaceous glands and neoplastic sebaceous lesions showed a distinctive dimorphic staining pattern with the antibody panel used. The central foamy 'sebaceous' cells expressed HMFG1 and EMA, but not PKK1 or MNF116, whereas the smaller, peripheral basal and ductal cells expressed PKK1 or MNF116 but not HMFG1 or EMA. CEA expression in sebaceous cells was unhelpful diagnostically. CONCLUSION: Normal sebaceous glands and all sebaceous neoplasms show a dimorphic cell population that can be identified using a small panel of antibodies on formalin-fixed, paraffin-embedded tissue. This distinctive staining pattern can be assessed retrospectively, even in small biopsies, and largely removes the need for fat stains on frozen sections to differentiate sebaceous lesions from other ocular neoplasms. The results also support the suggestion that ocular sebaceous neoplasms arise from a common stem cell, rather than from either sebaceous or basal/ductal cells.
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Authors | J S Johnson, J A Lee, D W Cotton, W R Lee, M A Parsons |
Journal | Eye (London, England)
(Eye (Lond))
Vol. 13 ( Pt 1)
Pg. 104-8
( 1999)
ISSN: 0950-222X [Print] England |
PMID | 10396393
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Formaldehyde
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Topics |
- Adenocarcinoma, Sebaceous
(diagnosis)
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(metabolism)
- Diagnosis, Differential
- Eyelid Neoplasms
(diagnosis)
- Female
- Formaldehyde
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Paraffin Embedding
- Retrospective Studies
- Sebaceous Gland Neoplasms
(diagnosis)
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