Properties of phage-receptor lipopolysaccharide from Pseudomonas morsprunorum.

Abstract

Lipopolysaccharide (LPS) of Pseudomonas morsprunorum was extracted with hot phenol and purified by repeated centrifuging followed by either block electrophoresis or gel filtration. LPS from a virulent isolate exhibited specific phage inactivation (PI50 = 0.05 mug LPS ml-1), whereas LPS from an avirulent phage-resistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulphate-cellulose acetate electrophoresis (pH 7.4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to I mg ml-1, but produced weak chlorosis in bean and tobacco at 2 mg ml-1: no visible symptoms appeared after treatment with lower concentrations. The chemical composition of the LPS was partly determined.

• Authors: A V Quirk, A Sletten, R C Hignett
• Journal: Journal of general microbiology (J Gen Microbiol) Vol. 96 Issue 2 Pg. 375-81 (Oct 1976) ISSN: 0022-1287 [Print] England
• PMID: 993780 (Publication Type: Journal Article)

Chemical References

• Lipopolysaccharides
• Polysaccharides, Bacterial

Topics

• Bacteriophages
• Lipopolysaccharides (analysis, pharmacology, physiology)
• Plants (drug effects)
• Polysaccharides, Bacterial (analysis, pharmacology, physiology)
• Pseudomonas (analysis)