[Tricalcium phosphate, an antibiotic carrier: a study focused on experimental osteomyelitis in rabbits].

Abstract

PURPOSE OF THE STUDY:

Macroporous beta tricalcium phosphate ceramic beads were elaborated to be a resorbable bone substitute and a drug delivery system carrying gentamicin or vancomycin. The aim of this study was to evaluate this implant into a rabbit experimental osteomyelitis.

MATERIAL AND METHOD:

Experimentation included 24 rabbits and was performed in three stages, according to Norden's description. Induction of osteomyelitis was obtained by injection of a sclerosing agent and of Staphylococcus aureus through the lateral side of the metaphysis of the proximal tibia. Three weeks after inoculation, animals were randomly dispatched to one of the three treatment groups. After surgical debridment (to collect cinetics data), the first group received no further treatment and was considered as a control; the second group received a ceramic implant; the third group received a gentamicin-loaded ceramic implant. Euthanasia occurred between 2 days and 21 days after the debridment. Bone samples were obtained to quantify the bacterial and gentamicin bone concentrations. Gentamicin level was also measured inside the ceramic implant. Antibiotic concentration was assessed by a immunoenzymatic method.

RESULTS:

Osteomyelitis was obtained in 21 of the 24 animals (87.5%). Antibiotic release was early and complete (before the third day) but gentamicin still remained in the bone for 10 days. Bacterial concentration suggested an antimicrobial activity of the implant, but not a full sterilisation of the osteomyelitis.

CONCLUSION:

Norden's experimental osteomyelitis model with rabbit was proposed to assess the therapeutic activity of systemic antibiotics, but not to evaluate biomaterials. Therefore we have shifted for experimental evaluation of biomaterials to a metaphyseal osteomyelitis in the sheep, the patterns of which are close from those of the human disease.

• Authors: J C Lambotte, H Thomazeau, G Cathelineau, G Lancien, J Minet, F Langlais
• Journal: Chirurgie; memoires de l'Academie de chirurgie (Chirurgie) Vol. 123 Issue 6 Pg. 572-9 (Dec 1998) ISSN: 0001-4001 [Print] France
• Vernacular Title: Phosphate tricalcique, vecteur d'antibiotiques: étude sur foyer d'ostéomyélite expérimentale chez le lapin.
• PMID: 9922597 (Publication Type: English Abstract, Journal Article)

Chemical References

• Anti-Bacterial Agents
• Biocompatible Materials
• Bone Substitutes
• Calcium Phosphates
• Drug Carriers
• Drug Implants
• Gentamicins
• beta-tricalcium phosphate
• tricalcium phosphate

Topics

• Animals
• Anti-Bacterial Agents (administration & dosage, pharmacokinetics)
• Biocompatible Materials (therapeutic use)
• Bone Substitutes (therapeutic use)
• Calcium Phosphates (therapeutic use)
• Ceramics
• Colony Count, Microbial
• Debridement
• Disease Models, Animal
• Drug Carriers
• Drug Delivery Systems
• Drug Implants
• Gentamicins (administration & dosage, pharmacokinetics)
• Humans
• Osteomyelitis (drug therapy, microbiology, surgery)
• Porosity
• Rabbits
• Random Allocation
• Sheep
• Staphylococcal Infections (drug therapy, surgery)
• Staphylococcus aureus (drug effects, growth & development)
• Tibia (drug effects, metabolism, microbiology)