Cultures of cells from fetal rat brain: methods to control composition, morphology, and biochemical activity.

Fetal tissue transplantation is a promising new approach for the treatment of neurodegenerative diseases, but the optimal conditions for preparing cells for transplantation have not been defined. The growth of a population of septal brain cells, primarily containing cholinergic neurons and glia, was characterized after seeding at densities from 5 x 10(4) to 6 x 10(5) cells/cm2, on polystyrene-, collagen-, laminin-, and fibronectin-coated surfaces, in the presence of serum and/or serum-free medium. Differentiated glial cells were selected by culture on fibronectin or laminin surfaces, in the presence of low amounts of serum (2.5% FBS) and G5, a soluble factor containing EGF and insulin. Differentiated neuronal cells were selected by culture on laminin, in the presence of low amounts of serum (2.5% FBS) and N2, a soluble factor containing supplemental hormones. In each case, a minimum seeding density of 1 x 10(5) cells/cm2 was required. Neuronal growth could be maintained long term (21 days) with high levels of neuronal activity (ChAT activity).
AuthorsM J Mahoney, W M Saltzman
JournalBiotechnology and bioengineering (Biotechnol Bioeng) Vol. 62 Issue 4 Pg. 461-7 (Feb 20 1999) ISSN: 0006-3592 [Print] UNITED STATES
PMID9921155 (Publication Type: Journal Article)
Chemical References
  • Culture Media
  • Choline O-Acetyltransferase
  • Animals
  • Biotechnology
  • Brain (cytology, enzymology)
  • Brain Tissue Transplantation
  • Cell Aggregation
  • Cell Culture Techniques (methods)
  • Cell Differentiation
  • Cells, Cultured
  • Choline O-Acetyltransferase (metabolism)
  • Culture Media
  • Fetal Tissue Transplantation
  • Fetus (cytology, enzymology)
  • Humans
  • Neurodegenerative Diseases (therapy)
  • Neuroglia (cytology, enzymology)
  • Neurons (cytology, enzymology)
  • Rats

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