Cultures of cells from fetal rat brain: methods to control composition, morphology, and biochemical activity.

Abstract

Fetal tissue transplantation is a promising new approach for the treatment of neurodegenerative diseases, but the optimal conditions for preparing cells for transplantation have not been defined. The growth of a population of septal brain cells, primarily containing cholinergic neurons and glia, was characterized after seeding at densities from 5 x 10(4) to 6 x 10(5) cells/cm2, on polystyrene-, collagen-, laminin-, and fibronectin-coated surfaces, in the presence of serum and/or serum-free medium. Differentiated glial cells were selected by culture on fibronectin or laminin surfaces, in the presence of low amounts of serum (2.5% FBS) and G5, a soluble factor containing EGF and insulin. Differentiated neuronal cells were selected by culture on laminin, in the presence of low amounts of serum (2.5% FBS) and N2, a soluble factor containing supplemental hormones. In each case, a minimum seeding density of 1 x 10(5) cells/cm2 was required. Neuronal growth could be maintained long term (21 days) with high levels of neuronal activity (ChAT activity).

• Authors: M J Mahoney, W M Saltzman
• Journal: Biotechnology and bioengineering (Biotechnol Bioeng) Vol. 62 Issue 4 Pg. 461-7 (Feb 20 1999) ISSN: 0006-3592 [Print] United States
• PMID: 9921155 (Publication Type: Journal Article)

Chemical References

• Culture Media
• Choline O-Acetyltransferase

Topics

• Animals
• Biotechnology
• Brain (cytology, enzymology)
• Brain Tissue Transplantation
• Cell Aggregation
• Cell Culture Techniques (methods)
• Cell Differentiation
• Cells, Cultured
• Choline O-Acetyltransferase (metabolism)
• Culture Media
• Fetal Tissue Transplantation
• Fetus (cytology, enzymology)
• Humans
• Neurodegenerative Diseases (therapy)
• Neuroglia (cytology, enzymology)
• Neurons (cytology, enzymology)
• Rats