HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

The etiology of genital ulcer disease by multiplex polymerase chain reaction and relationship to HIV infection among patients attending sexually transmitted disease clinics in Pune, India.

AbstractOBJECTIVES:
To determine the etiology of genital ulcer disease (GUD) among patients attending sexually transmitted disease (STD) clinics in Pune, India, and to examine the relationship to HIV infection and compare the clinical diagnosis of GUD with the results of a multiplex polymerase chain reaction (M-PCR) assay for Treponema pallidum, herpes simplex virus (HSV), and Hemophilus ducreyi infection.
METHODS:
Between June 20, 1994, and September 26, 1994, 302 patients with a genital ulcer were evaluated. Clinical etiology of GUD was based on physical appearance and microbiologic evaluations which included darkfield microscopy and serology for syphilis. Swabs of each genital ulcer were tested for HSV antigen by enzyme immunoassay (Herpchek; Dupont, Wilmington, DE) and processed in a multiplex PCR assay (M-PCR; Roche, Branchburg, NJ) for simultaneous detection of HSV, Treponema pallidum, and Hemophilus ducreyi.
RESULTS:
Two hundred seventy-seven men and 25 women with a median age of 25 were evaluated. The seroprevalence of HIV was 22.2%. The etiology of GUD as determined by M-PCR was HSV (26%), H. ducreyi (23%), T. pallidum (10%), and multiple infections (7%); no etiology was identified in 34%. HIV seroprevalence was higher among those patients positive for HSV compared with other etiologies (OR = 2.1, CI: 1.2-3.7; p = 0.01). When compared with M-PCR, the Herpchek test was 68.5% sensitive and 99.5% specific. Darkfield detection for T. pallidum was 39% sensitive and 82% specific, in contrast to rapid plasma reagin and fluorescent treponemal antibody absorption test, which was 66% sensitive and 90% specific. Clinical diagnosis alone or in combination with basic laboratory tests showed poor agreement with M-PCR.
AuthorsA Risbud, K Chan-Tack, D Gadkari, R R Gangakhedkar, M E Shepherd, R Bollinger, S Mehendale, C Gaydos, A Divekar, A Rompalo, T C Quinn
JournalSexually transmitted diseases (Sex Transm Dis) Vol. 26 Issue 1 Pg. 55-62 (Jan 1999) ISSN: 0148-5717 [Print] United States
PMID9918324 (Publication Type: Journal Article)
Topics
  • Adolescent
  • Adult
  • Chancroid (complications, diagnosis, epidemiology)
  • Female
  • Genital Diseases, Female (complications, diagnosis, etiology)
  • Genital Diseases, Male (complications, diagnosis, etiology)
  • HIV Infections (complications, epidemiology)
  • Herpes Simplex (complications, diagnosis, epidemiology)
  • Humans
  • India (epidemiology)
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Sexually Transmitted Diseases (complications, diagnosis, etiology)
  • Syphilis (complications, diagnosis, epidemiology)
  • Ulcer (complications, diagnosis, etiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: