Abstract | OBJECTIVE: Previous investigations have shown the effectiveness of sustained intra- or extravascular administration of vascular endothelial growth factor ( VEGF) in chronic myocardial ischemia in improvement of left ventricular function. The present investigations were undertaken in order to evaluate efficacy of a single bolus or local intracoronary delivery. METHODS: Yorkshire pigs underwent placement of a left circumflex artery ameroid occluder. Three weeks later the animals were randomized to treatment with VEGF (20 micrograms) accomplished by local intracoronary delivery system (InfusaSleeve, n = 10), intracoronary bolus infusion (n = 7) or by epicardial implantation of an osmotic delivery system (n = 7). An additional group of animals received intracoronary administration of saline and served as a control (n = 9). Three weeks after initiation of therapy, the animals were evaluated with regard to myocardial perfusion and global as well as regional ventricular function. RESULTS: All three VEGF treatment groups but not the control animals demonstrated a significant increase in the left-to-left (but not right-to-left) collateral index, myocardial blood flow (pre- therapy LCX vs. LAD (average of all groups): 0.76 +/- 0.35 vs. 0.96 +/- 0.38 ml*min-1*g-1, p = 0.03; post- therapy: LCX vs. LAD: 1.16 +/- 0.39 vs. 1.15 +/- 0.28 ml*min-1*g-1, p = NS) and coronary vasodilatory reserve 3 weeks after growth factor administration. The observed increase in VEGF-induced perfusion correlated with improvement in regional ventricular function in all VEGF-treated groups (pre- therapy vs. post- therapy: i.c. VEGF 20 +/- 5.1 vs. 33 +/- 4.8; local VEGF 16 +/- 2.8 vs. 33.6; pump VEGF 17 +/- 3.8 vs. 34 +/- 4.9 p < 0.05 for all) but not control animals (21 +/- 3.3 vs. 27 +/- 5.8, p = NS). CONCLUSION: Single intracoronary delivery (intravascular bolus or local delivery) of VEGF is effective in stimulating physiologically significant angiogenesis in porcine model of chronic myocardial ischemia.
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Authors | J J Lopez, R J Laham, A Stamler, J D Pearlman, S Bunting, A Kaplan, J P Carrozza, F W Sellke, M Simons |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 40
Issue 2
Pg. 272-81
(Nov 1998)
ISSN: 0008-6363 [Print] England |
PMID | 9893720
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Endothelial Growth Factors
- Lymphokines
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
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Topics |
- Animals
- Chronic Disease
- Coronary Angiography
- Coronary Circulation
(drug effects)
- Endothelial Growth Factors
(administration & dosage, therapeutic use)
- Infusion Pumps, Implantable
- Infusions, Intra-Arterial
- Injections, Intra-Arterial
- Lymphokines
(administration & dosage, therapeutic use)
- Magnetic Resonance Imaging
- Myocardial Ischemia
(diagnostic imaging, drug therapy, physiopathology)
- Random Allocation
- Statistics, Nonparametric
- Swine
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
- Ventricular Function, Left
(drug effects)
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