Abstract |
Fibroblast growth factors (FGFs) have mitogenic activity toward a wide variety of cells of mesenchymal, neuronal, and epithelial origin and regulate events in normal embryonic development, angiogenesis, wound repair, and neoplasia. FGF-2 is expressed in many normal adult tissues and can regulate migration and replication of intestinal epithelial cells in culture. However, little is known about the effects of FGF-2 on intestinal epithelial stem cells during either normal epithelial renewal or regeneration of a functional epithelium after injury. In this study, we investigated the expression of FGF-2 in the mouse small intestine after irradiation and determined the effect of exogenous FGF-2 on crypt stem cell survival after radiation injury. Expression of FGF-2 mRNA and protein began to increase at 12 h after gamma-irradiation, and peak levels were observed from 48 to 120 h after irradiation. At all times after irradiation, the higher molecular mass isoform ( approximately 24 kDa) of FGF-2 was the predominant form expressed in the small intestine. Immunohistochemical analysis of FGF-2 expression after radiation injury demonstrated that FGF-2 was predominantly found in the mesenchyme surrounding regenerating crypts. Exogenous recombinant human FGF-2 (rhFGF-2) markedly enhanced crypt stem cell survival when given before irradiation. We conclude that expression of FGF-2 is induced by radiation injury and that rhFGF-2 can enhance crypt stem cell survival after subsequent injury.
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Authors | C W Houchen, R J George, M A Sturmoski, S M Cohn |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 276
Issue 1
Pg. G249-58
(01 1999)
ISSN: 0002-9513 [Print] United States |
PMID | 9887002
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- RNA, Messenger
- Recombinant Proteins
- Fibroblast Growth Factor 2
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Topics |
- Animals
- Cell Survival
(drug effects)
- Female
- Fibroblast Growth Factor 2
(genetics, metabolism, pharmacology)
- Humans
- Intestines
(drug effects, pathology, radiation effects)
- Mice
- Mice, Inbred Strains
- RNA, Messenger
(metabolism)
- Radiation Injuries, Experimental
(metabolism)
- Recombinant Proteins
- Stem Cells
(drug effects, physiology)
- Tissue Distribution
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