Abstract | OBJECTIVE: DESIGN: MATERIALS AND METHODS:
Systolic hypertension was induced within 3 weeks in male Sprague-Dawley rats fed on a fructose-rich diet. After continual monitoring of blood pressure and plasma insulin concentrations, the animals were killed at the end of experiment to determine plasma levels of ET-1, the contractile response of aortic rings to ET-1, and ET-1 and ET(A) receptor gene expressions. In a separate experiment, BQ-610 was administered to lower the effect of ET-1 in rats with fructose-induced hypertension. RESULTS: Compared with control rats given normal chow, the fructose-fed rats developed systolic hypertension after 3 weeks of the diet (127+/-3.7 versus 110+/-5.5 mmHg, P < 0.01) and hyperinsulinemia both before (1 07.1+/-32.5 versus 48.5+/-14.3 pmol/l, P < 0.005) and after (96.6+/-63.7 versus 50.4+/-5.6 pmol/l, P< 0.05) they became hypertensive. Although plasma ET-1 levels did not differ between the rat groups, aortic ring contraction-concentration curves, indicating vessel contractility in response to ET-1, were significantly greater in these rats than in controls (F1,72 = 12.34, P< 0.00077). Messenger RNA extracted from the tail arteries and blotted with both ET-1 and ET(A) probes showed that fructose-fed rats had greater ET-1 and ET(A)-receptor gene expression than control rats. Concomitant administration of BQ-610 to rats fed on a fructose diet significantly reduced the hypertension. Conclusions These findings suggest that elevated vascular expression of ET-1 and ET(A) receptor genes may mediate the development of hypertension and hyperinsulinemia in rats fed a fructose-rich diet
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Authors | C C Juan, V S Fang, Y P Hsu, Y J Huang, D B Hsia, P C Yu, C F Kwok, L T Ho |
Journal | Journal of hypertension
(J Hypertens)
Vol. 16
Issue 12 Pt 1
Pg. 1775-82
(Dec 1998)
ISSN: 0263-6352 [Print] England |
PMID | 9869011
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Dietary Carbohydrates
- Endothelin-1
- Insulin
- Oligopeptides
- Receptor, Endothelin A
- Receptors, Endothelin
- BQ 610
- Fructose
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Topics |
- Animals
- Arteries
(chemistry, cytology)
- Blood Glucose
(metabolism)
- Blood Pressure
(drug effects)
- Dietary Carbohydrates
(pharmacology)
- Disease Models, Animal
- Endothelin-1
(biosynthesis, blood, genetics)
- Fructose
(pharmacology)
- Hypertension
(chemically induced, metabolism)
- Insulin
(blood)
- Male
- Muscle, Smooth, Vascular
(cytology, metabolism)
- Oligopeptides
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Receptor, Endothelin A
- Receptors, Endothelin
(biosynthesis, genetics)
- Tail
(chemistry, cytology)
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