Enhancement of XPG mRNA transcription by human interferon-beta in Cockayne syndrome cells with complementation group B.

Abstract

Human interferon-beta (HuIFN-beta) confers UV-refractoriness in association with increased DNA repair capacity to human cells. We examined the modulation of XPG gene expression by HuIFN-beta in UV-sensitive cells from Cockayne syndrome complementation B (CSB), xeroderma pigmentosum complementation A (XPA) and normal control cells. Northern blot analysis revealed that XPG mRNA was more extensively transcribed in CSB cells treated with HuIFN-beta than in those without HuIFN-beta treatment. XPG mRNA from XPA cells and normal control cells was not markedly transcribed by HuIFN-beta treatment compared to that from CSB cells. The findings suggested that different mechanisms of UV-refractoriness by HuIFN-beta exist between CS and XP.

• Authors: Y Suzuki, K Sugita, N Suzuki, K Kita, Y Higuchi, A Yamaura, Y Kohno
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 3 Issue 1 Pg. 87-9 (Jan 1999) ISSN: 1107-3756 [Print] Greece
• PMID: 9864391 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA excision repair protein ERCC-5
• DNA-Binding Proteins
• Nuclear Proteins
• RNA, Messenger
• Transcription Factors
• RNA
• Interferon-beta
• Endonucleases

Topics

• Blotting, Northern
• Cell Line, Transformed
• Cockayne Syndrome (genetics, pathology)
• DNA-Binding Proteins (genetics)
• Endonucleases
• Humans
• Interferon-beta (pharmacology)
• Nuclear Proteins
• RNA (analysis, genetics)
• RNA, Messenger (genetics)
• Transcription Factors
• Transcription, Genetic (drug effects)