Response to reactive nitrogen intermediates in Mycobacterium tuberculosis: induction of the 16-kilodalton alpha-crystallin homolog by exposure to nitric oxide donors.

In contrast to the apparent paucity of Mycobacterium tuberculosis response to reactive oxygen intermediates, this organism has evolved a specific response to nitric oxide challenge. Exposure of M. tuberculosis to NO donors induces the synthesis of a set of polypeptides that have been collectively termed Nox. In this work, the most prominent Nox polypeptide, Nox16, was identified by immunoblotting and by N-terminal sequencing as the alpha-crystallin-related, 16-kDa small heat shock protein, sHsp16. A panel of chemically diverse donors of nitric oxide, with the exception of nitroprusside, induced sHsp16 (Nox16). Nitroprusside, a coordination complex of Fe2+ with a nitrosonium (NO+) ion, induced a 19-kDa polypeptide (Nox19) homologous to the nonheme bacterial ferritins. We conclude that the NO response in M. tuberculosis is dominated by increased synthesis of the alpha-crystallin homolog sHsp16, previously implicated in stationary-phase processes and found in this study to be a major M. tuberculosis protein induced upon exposure to reactive nitrogen intermediates.
AuthorsT R Garbe, N S Hibler, V Deretic
JournalInfection and immunity (Infect Immun) Vol. 67 Issue 1 Pg. 460-5 (Jan 1999) ISSN: 0019-9567 [Print] UNITED STATES
PMID9864257 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antigens, Bacterial
  • Crystallins
  • Heat-Shock Proteins
  • Nitric Oxide Donors
  • Nitric Oxide
  • Ferritins
  • Amino Acid Sequence
  • Antigens, Bacterial (chemistry)
  • Crystallins (biosynthesis, chemistry)
  • Ferritins (chemistry)
  • Gene Expression Regulation, Bacterial (drug effects)
  • Heat-Shock Proteins (chemistry)
  • Molecular Sequence Data
  • Molecular Weight
  • Mycobacterium tuberculosis (drug effects, genetics, metabolism)
  • Nitric Oxide (metabolism)
  • Nitric Oxide Donors (metabolism, pharmacology)
  • Sequence Homology, Amino Acid

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