Abstract |
The gene that suppresses the phenotype of the cpz-2 mutation, which results in changing the sensitivity to chlorpromazine in relation to mycelial growth and circadian rhythms, was cloned in Neurospora crassa. This gene is not the cpz-2 gene itself but rather is identical to the spe-3 gene that encodes spermidine synthase in Neurospora. The intracellular content of spermidine was lowered in the cpz-2 strain compared to that of the wild-type strain. By integration of the spe-3 gene or by the addition of spermidine into culture medium, the temperature sensitivity of mycelial growth was lost and the conidiation rhythm became sensitive to chlorpromazine in the cpz-2 strain, as was observed in the wild-type strain, but the hypersensitivity of mycelial growth on chlorpromazine in the cpz-2 strain was not affected. Therefore, it appears that spermidine determines only the sensitivity of the conidiation rhythm to chlorpromazine.
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Authors | S Katagiri, K Onai, H Nakashima |
Journal | Journal of biological rhythms
(J Biol Rhythms)
Vol. 13
Issue 6
Pg. 452-60
(Dec 1998)
ISSN: 0748-7304 [Print] United States |
PMID | 9850006
(Publication Type: Journal Article)
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Chemical References |
- Calmodulin
- DNA, Fungal
- Dopamine Antagonists
- Chlorpromazine
- Spermidine
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Topics |
- Amino Acid Sequence
- Base Sequence
- Calmodulin
(antagonists & inhibitors)
- Chlorpromazine
(pharmacology)
- Circadian Rhythm
(drug effects)
- DNA, Fungal
(biosynthesis, genetics)
- Dopamine Antagonists
(pharmacology)
- Gene Library
- Molecular Sequence Data
- Neurospora crassa
(drug effects, growth & development)
- Phenotype
- Spermidine
(pharmacology)
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