Direct activation of human CD8+ cytotoxic T lymphocytes by interleukin-18.

Abstract

Direct activation of human cytotoxic T lymphocytes (CTL) by interleukin (IL)-18 was observed in a system in which CTL effective against autologous tumor cells were generated. Peripheral blood mononuclear cells (PBMC) from tumor-bearing patients, after removal of natural killer (NK) cells, were cultured in a medium containing IL-1, -2, -4, and -6, with or without IL-18, and stimulated with autologous tumor cells. IL-18 increased the activity of the CTL and the proportion of autologous CD8+ T cells present after 28 days in the induction culture. When purified CD8+ T cells were cultured in the presence of IL-18 and IL-2 for 7 days, the CTL showed enhanced cytotoxic activity against autologous tumor cells. Moreover, a purified CD8+ T cell population, which did not exhibit any apparent cytotoxic activity against autologous tumor cells, displayed cytotoxic activity after 7-day incubation with IL-18. These results suggest that IL-18 may be useful to generate autologous CTL in humans and may thereby contribute to adoptive immunotherapy for tumors.

• Authors: M Kohyama, K Saijyo, M Hayasida, T Yasugi, M Kurimoto, T Ohno
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 89 Issue 10 Pg. 1041-6 (Oct 1998) ISSN: 0910-5050 [Print] Japan
• PMID: 9849583 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1
• Interleukin-18
• Interleukin-2
• Interleukin-6
• Interleukins
• Recombinant Proteins
• Interleukin-4

Topics

• Adenocarcinoma
• Carcinoma, Renal Cell
• Cells, Cultured
• Coculture Techniques
• Cytotoxicity, Immunologic
• Glioblastoma
• Humans
• Interleukin-1 (pharmacology)
• Interleukin-18 (pharmacology)
• Interleukin-2 (pharmacology)
• Interleukin-4 (pharmacology)
• Interleukin-6 (pharmacology)
• Interleukins (pharmacology)
• Kidney Neoplasms
• Lymphocyte Activation (drug effects)
• Neoplasms (immunology)
• Recombinant Proteins (pharmacology)
• Stomach Neoplasms
• T-Lymphocytes, Cytotoxic (drug effects, immunology)
• Tumor Cells, Cultured