Abstract |
We have previously shown that infection of CD4(+) T lymphocytes with the T-lymphotropic human herpesvirus 7 (HHV-7) downregulates surface CD4, which represents the high-affinity receptor for HHV-7. In this study, we report that HHV-7 infection also causes a progressive loss of the surface CXC-chemokine receptor 4 (CXCR4) in CD4(+) T cells, accompanied by a reduced intracellular Ca2+ flux and chemotaxis in response to stromal cell-derived factor-1 (SDF-1), the specific CXCR4 ligand. Moreover, CXCR4 is downregulated from the surface of HHV-7-infected T cells independently of CD4. Because intracellular CXCR4 antigen and mRNA levels are unaffected in productively HHV-7-infected cells, the downregulation of CXCR4 apparently does not involve a transcritional block. Since CXCR4 functions in association with CD4 to permit entry of several human immunodeficiency virus (HIV) isolates, the potential of HHV-7 to persistently downregulate the surface expression of CXCR4 may provide novel strategies for limiting HIV infection.
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Authors | P Secchiero, D Zella, O Barabitskaja, M S Reitz, S Capitani, R C Gallo, G Zauli |
Journal | Blood
(Blood)
Vol. 92
Issue 12
Pg. 4521-8
(Dec 15 1998)
ISSN: 0006-4971 [Print] United States |
PMID | 9845516
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD4 Antigens
- CXCL12 protein, human
- Chemokine CXCL12
- Chemokines, CXC
- RNA, Messenger
- Receptors, CXCR4
- Calcium
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Topics |
- CD4 Antigens
(metabolism)
- CD4-Positive T-Lymphocytes
(drug effects, metabolism, physiology, virology)
- Calcium
(metabolism)
- Cell Line
- Cells, Cultured
- Chemokine CXCL12
- Chemokines, CXC
(pharmacology)
- Chemotaxis
(drug effects)
- Down-Regulation
(immunology)
- Flow Cytometry
- Fluorescent Antibody Technique, Indirect
- HIV Infections
(immunology)
- HIV-1
(pathogenicity)
- Herpesviridae Infections
(immunology)
- Herpesvirus 7, Human
(pathogenicity)
- Humans
- Intracellular Fluid
(metabolism)
- RNA, Messenger
(analysis)
- Receptors, CXCR4
(genetics, metabolism)
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