Accurate amniotic fluid bilirubin analysis from the "bloody tap". A preliminary report.

Abstract

The quantitation of bilirubin in amniotic fluid is of paramount importance in prognosticating the severity of Rh isoimmunization. Amniotic fluid contaminated by maternal blood can result in erroneous results when direct spectrophotemetric analysis is performed on such fluid. The technique and results of a one-step chloroform extraction performed on "bloody" amniotic fluid is presented. Results confirm that chloroform extraction yields more accurate clinical information in regard to Rh isoimmunized patients in whom a "bloody tap" is obtained.

• Authors: C J Hochberg, A P Witheiler, H Cook
• Journal: American journal of obstetrics and gynecology (Am J Obstet Gynecol) Vol. 126 Issue 5 Pg. 531-4 (Nov 01 1976) ISSN: 0002-9378 [Print] United States
• PMID: 984122 (Publication Type: Journal Article)

Chemical References

• Chloroform
• Bilirubin

Topics

• Amniocentesis
• Amniotic Fluid (analysis)
• Bilirubin (analysis)
• Blood
• Chloroform
• Erythroblastosis, Fetal (diagnosis)
• Female
• Humans
• Infant, Newborn
• Methods
• Pregnancy
• Spectrophotometry