Accelerated viraemia in cats vaccinated with fixed autologous FIV-infected cells.

Abstract

We have vaccinated cats with fixed autologous FIV infected PBMC to determine whether autologous presentation of antigen is capable of inducing a protective immune response against homologous challenge. To this end autologous PBMC were infected with a FIV molecular clone (19k1). When infection was established, cells were inactivated by dialysis against paraformaldehyde. Upon vaccination, cats developed a virus specific immune response as measured by ELISA against the Gag protein of FIV. No antibodies against the envelope protein were detected with a peptide ELISA. Virus neutralizing antibodies however could be detected with a neutralization assay based on infection of CrFK cells, but not in an assay based on infection of primary T-cells. Although vaccination led to the induction of these virus-specific immune responses, vaccinated cats were not protected against homologous challenge but showed an accelerated viraemia upon infection. This was shown both by PCR and cell-associated viral load. The possible mechanisms underlying this observation are discussed in this paper.

• Authors: J A Karlas, K H Siebelink, M A v Peer, W Huisman, G F Rimmelzwaan, A D Osterhaus
• Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 65 Issue 2-4 Pg. 353-65 (Oct 23 1998) ISSN: 0165-2427 [Print] Netherlands
• PMID: 9839884 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral
• DNA Primers
• DNA, Viral
• Viral Vaccines

Topics

• Animals
• Antibodies, Viral (analysis)
• Blood Component Transfusion
• Blood Transfusion, Autologous
• Cats
• DNA Primers (chemistry)
• DNA, Viral (analysis)
• Feline Acquired Immunodeficiency Syndrome (immunology, prevention & control)
• Immunodeficiency Virus, Feline (genetics, immunology)
• Polymerase Chain Reaction (veterinary)
• T-Lymphocytes (immunology, virology)
• Vaccination (veterinary)
• Viral Load
• Viral Vaccines (administration & dosage)
• Viremia (etiology, immunology)