The role of alpha2 beta1 and alpha3 beta1 integrin receptors in the initial anchoring of MDA-MB-231 human breast cancer cells to cortical bone matrix.

Abstract

Molecular mechanisms involved in the rapid attachment of the human breast cancer cell line MDA-MD-231 to cortical bone matrix were studied. The attachment of MDA-MD-231 cells to cortical bone disks could be blocked by 75% when cells were pretreated with a monoclonal antibody to the beta1-subunit of the integrin family. Monoclonal antibodies against the alpha2, alpha3, and alpha5 integrin subunits inhibited the attachment by 76, 26, and 8 % respectively. Collagenase type I and collagen type I antibody blocked the cell attachment by 45 and 50 % whereas pretreatment of the cells with soluble collagen type I blocked the attachment by 85 %. Our study with a panel of cancer cell lines further showed a close correlation between alpha2 beta1 and alpha3 beta1 integrin receptor expression and the capability to attach to cortical bone. These receptors appear to be the key receptors utilized by cancer cells for the initial attachment to cortical bone, and this could facilitate the localization of alpha2 beta1 and alpha3 beta1 expressing cancer cells to the skeleton.

• Authors: A Lundström, J Holmbom, C Lindqvist, T Nordström
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 250 Issue 3 Pg. 735-40 (Sep 29 1998) ISSN: 0006-291X [Print] United States
• PMID: 9784415 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 1998 Academic Press.

Chemical References

• Integrin alpha3beta1
• Integrins
• Receptors, Collagen
• Collagenases

Topics

• Animals
• Bone Matrix (pathology)
• Breast Neoplasms (pathology)
• Cattle
• Cell Adhesion (drug effects, physiology)
• Collagenases (pharmacology)
• Female
• Humans
• Integrin alpha3beta1
• Integrins (physiology)
• Receptors, Collagen
• Tumor Cells, Cultured