Abstract |
We related events in immunohistochemical studies on formalin-fixed, paraffin-embedded preparations of 146 colorectal cancer patients. One hundred (68.5%) revealed cytoplasmic staining and only 3 of the 100 showed membranous staining. Western blot analysis revealed proteins with molecular masses of 185 and 155 kD following immunohistochemical membranous and cytoplasmic staining, but only a 155-kD protein following cytoplasmic staining. Amplification of the c-erbB-2 gene was detected in 2 of 44 cases (4.5%). Cytoplasmic c-erbB-2 overexpression correlated with tumor size (p < 0.01), subserosal invasion (p < 0.05), liver metastasis (p < 0.01) and Dukes' classification. Overall survival rates and survival rates for Dukes'B patients were significantly lower in the group with cytoplasmic c-erbB-2 overexpression than in the group without cytoplasmic c-erbB-2 overexpression. Multivariate regression analysis showed cytoplasmic c-erbB-2 overexpression to be an independent prognostic factor for colorectal cancer. These results suggest that overexpression of cytoplasmic c-erbB-2 protein plays an important role in the progression of colorectal cancer and is considered to be an independent prognostic indicator of this lesion.
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Authors | T Osako, M Miyahara, S Uchino, M Inomata, S Kitano, M Kobayashi |
Journal | Oncology
(Oncology)
1998 Nov-Dec
Vol. 55
Issue 6
Pg. 548-55
ISSN: 0030-2414 [Print] Switzerland |
PMID | 9778622
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adenocarcinoma
(chemistry, metabolism, pathology)
- Adult
- Aged
- Aged, 80 and over
- Blotting, Western
- Colorectal Neoplasms
(chemistry, metabolism, pathology)
- Female
- Gene Amplification
- Genes, erbB-2
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Neoplasm Staging
- Prognosis
- Proportional Hazards Models
- Receptor, ErbB-2
(analysis, biosynthesis)
- Survival Analysis
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