Suppressive effects of SP-A on ionomycin-induced IL-8 production and release by eosinophils.

Abstract

Recent studies have demonstrated that pulmonary surfactant protein (SP)-A plays a potential role in modifying inflammation and immune function. To see whether SP-A could modify IL-8 production and release by eosinophils stimulated with ionomycin, SP-A purified from surfactant recovered from patients with alveolar proteinosis was added to eosinophils isolated by the negative-selection method with immunomagnetic beads, and cultured for 24 h. The concentrations of IL-8 in the cell-free supernatants and cell lysates were then measured by ELISA. SP-A attenuated the production of IL-8 by eosinophils in a concentration-dependent manner. SP-A also attenuated the release of IL-8 from the eosinophils. The addition of SP-A antibody (PE10) reversed these effects of SP-A completely. These data suggest that SP-A may have the potential to modify allergic inflammation by inhibiting the release and production of IL-8 by eosinophils.

• Authors: G Cheng, T Ueda, H Nakajima, A Nakajima, S Kinjyo, S Motojima, T Fukuda
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 117 Suppl 1 Pg. 59-62 (Sep 1998) ISSN: 1018-2438 [Print] Switzerland
• PMID: 9758900 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Interleukin-8
• Proteolipids
• Pulmonary Surfactant-Associated Protein A
• Pulmonary Surfactant-Associated Proteins
• Pulmonary Surfactants
• Ionomycin

Topics

• Antibodies, Monoclonal (pharmacology)
• Asthma (drug therapy, etiology, immunology)
• Dose-Response Relationship, Drug
• Eosinophils (drug effects, immunology)
• Humans
• In Vitro Techniques
• Inflammation (drug therapy, etiology, immunology)
• Interleukin-8 (biosynthesis, metabolism)
• Ionomycin (pharmacology)
• Lung (immunology, metabolism)
• Proteolipids (administration & dosage, antagonists & inhibitors, pharmacology)
• Pulmonary Surfactant-Associated Protein A
• Pulmonary Surfactant-Associated Proteins
• Pulmonary Surfactants (administration & dosage, antagonists & inhibitors, pharmacology)