Alterations in [3H]L-N(G)-nitroarginine binding in brain after transient global or transient focal ischemia in gerbils and rats.

Abstract

We investigated the post-ischemic change in [3H]L-N(G)-nitroarginine binding as a marker of nitric oxide (NO) synthase in the animal brain after transient global ischemia or transient focal ischemia. Transient global ischemia in gerbils was induced for 10 min followed by 1 h to 7 days of recirculation. Transient focal ischemia in rats was induced for 45 min followed by 3 days of recirculation. Following transient global ischemia, [3H]L-N(G)-nitroarginine binding showed a significant increase in the striatum (17-18%) and hippocampal CA1 sector (24%) at 48 and 24 h after recirculation, respectively. The hippocampal CA3 sector also showed a significant elevation (32-40%) in [3H]L-N(G)-nitroarginine binding at 24 and 48 h after global ischemia. Furthermore, the dentate gyrus showed a significant increase (30-32%) in [3H]L-N(G)-nitroarginine binding at 5, 24 and 48 h after global ischemia. Thereafter, a significant reduction in [3H]L-N(G)-nitroarginine binding was observed only in the dentate gyrus 7 days after recirculation. In contrast, [3H]L-N(G)-nitroarginine binding was unchanged in the thalamus throughout the recirculation periods. Histological analysis revealed that transient global ischemia caused severe damage or cellular damage in the striatum and the hippocampal CA1 sector. The hippocampal CA3 sector and thalamus were mildly damaged, whereas the dentate gyrus was morphologically intact. Following transient focal ischemia, a marked elevation (50-52%) in [3H]L-N(G)-nitroarginine binding was found in the regions of the ipsilateral striatum in which severe infarction occurred. Our findings suggest that [3H]L-N(G)-nitroarginine binding increases in the striatum and hippocampus after transient global ischemia or transient focal ischemia. This increase in [3H]L-N(G)-nitroarginine binding may play a pivotal role not only in the pathogenesis of ischemic brain damage, but also in the restoration of injury areas after cerebral ischemia.

• Authors: T Araki, H Kato, K Shuto, Y Itoyama
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 354 Issue 2-3 Pg. 153-9 (Aug 07 1998) ISSN: 0014-2999 [Print] Netherlands
• PMID: 9754915 (Publication Type: Journal Article)

Chemical References

• Radiopharmaceuticals
• Tritium
• Nitroarginine
• Nitric Oxide Synthase

Topics

• Animals
• Autoradiography
• Brain (diagnostic imaging, enzymology, metabolism, pathology)
• Gerbillinae
• Ischemic Attack, Transient (enzymology, metabolism, pathology)
• Male
• Nitric Oxide Synthase (metabolism)
• Nitroarginine (pharmacokinetics)
• Radionuclide Imaging
• Radiopharmaceuticals (pharmacokinetics)
• Rats
• Rats, Wistar
• Tritium