Tyrosyl-, histidyl-, lysyl-, and phenylalanyl-
tRNA's from 3
tumors (DBAH, DBAH, and DBA), differing in growth rates and from host mammary glands and liver, were compared by means of methylated
albumin kieselguhr (MAK) column and by reverse-phase-5 chromatography. The elution profiles of lysyl-
tRNA's from DBAH and DBA, phenylalanyl
Trna's from DBAH and DBAH, and histidyl-
tRNA's from DBA3
tumors exhibited extra isoaccepting species, compared with host liver and mammary glands. The distribution of acylatable tyrosyl-
tRNA's in DBA3 and DBAH, phenylalanyl-
tRNA's in DBAH and DBAH2, and histid-l-
tRNA's in DBA3 is higher than that in liver, whereas no appreciable differences were observed in the lysyl-
tRNA contents of the
tumors and liver. The chromatographic alternations appeared to be a property of the
tumor tRNA's and not due to differences in aminoacyl-
tRNA synthetases or due to the aggregation of
tRNA's. The structural and functional significance of these findings are discussed.