Development of a sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method for the measurement of 7-cyanoquinocarcinol in human plasma.

Abstract

A sensitive method for the determination of an anti-cancer agent, DX-52-1 (7-cyanoquinocarcinol, I) and quinocarmycin (II) which is formed from I either by metabolism or degradation, in human plasma has been developed utilising liquid chromatography electrospray-ionization tandem mass spectrometry (LC-ESI-MS-MS). The procedure involves solid-phase extraction at pH 2 and low temperature (4-6 degrees C) to prevent the decomposition of I to II, the separation by reversed-phase HPLC and the multiple reaction monitoring (MRM) by ESI-MS-MS. The mean precision and accuracy at the lower limit of quantitation (LLOQ) of I, 0.25 ng ml(-1), were 8.7% and - 10.8%, respectively. Since an interfering peak eluting slightly earlier than II was observed on the HPLC of blank plasma, the LLOQ of II was set at 5 ng ml(-1) where the mean precision and accuracy were 15.6% and -9.8%. The results suggested that the method is useful for the simultaneous monitoring of I and II in the clinical trials of I.

• Authors: K Yamaguchi, E Fuse, M Takashima, T Yasuzawa, T Kuwabara, S Kobayashi
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 713 Issue 2 Pg. 447-51 (Aug 25 1998) ISSN: 1387-2273 [Print] Netherlands
• PMID: 9746263 (Publication Type: Journal Article)

Chemical References

• Antibiotics, Antineoplastic
• Isoquinolines
• DX 52-1
• quinocarcin

Topics

• Antibiotics, Antineoplastic (blood)
• Chromatography, Liquid
• Humans
• Isoquinolines (blood)
• Male
• Mass Spectrometry
• Sensitivity and Specificity