Molecular cloning and characterization of a tumor rejection antigen from rat histiocytoma, AK-5.

Abstract

We have cloned and sequenced a tumor rejection antigen from a highly immunogenic rat histiocytoma, AK-5. Recombinant antigen induced an antibody response in syngeneic hosts, and the immunized animals showed significant resistance to AK-5 tumor challenge, where growth of the tumor was retarded. Autologous anti-AK-5 antibody, as well as the specific monoclonal antibodies raised against whole AK-5 cells, recognized the recombinant protein. Similarly, recombinant antigen was able to neutralize antitumor antibody in a complement-mediated lysis assay. Antibodies raised against the fusion protein recognized a 60-kDa protein from AK-5 cell extracts. A cDNA sequence analysis revealed significant homology with mouse thymus (81%), lymph node (83%), and human brain (71%) cDNAs; however, the function of these genes is not known. These observations suggest the recombinant protein to be a strong candidate for the tumor rejection antigen that is involved in the spontaneous regression of the AK-5 tumor in syngeneic hosts.

• Authors: T Muralikrishna, Z Begum, C V Swamy, A Khar
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 17 Issue 7 Pg. 603-12 (Jul 1998) ISSN: 1044-5498 [Print] United States
• PMID: 9703019 (Publication Type: Journal Article)

Chemical References

• AK-5 antigen
• Antibodies, Neoplasm
• Antigens, Neoplasm
• DNA, Complementary
• DNA, Neoplasm
• Recombinant Fusion Proteins
• Glutathione Transferase

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Neoplasm (genetics, immunology)
• Antigens, Neoplasm (analysis, genetics, immunology)
• Base Sequence
• Cloning, Molecular
• DNA, Complementary (chemistry, genetics)
• DNA, Neoplasm (genetics, isolation & purification)
• Female
• Genes (genetics)
• Glutathione Transferase (immunology)
• Male
• Molecular Sequence Data
• Neutralization Tests
• Rats
• Rats, Wistar
• Recombinant Fusion Proteins (genetics, immunology)
• Sequence Alignment
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Sequence Homology, Nucleic Acid
• Tumor Cells, Cultured (chemistry, immunology, metabolism)