Abstract | BACKGROUND: During recovery from intestinal ischemic injury, there is rapid growth of intestinal epithelia with regeneration of damaged villi. This study examines the effects of heparin-binding EGF-like growth factor ( HB-EGF) on the recovery of intestinal epithelial cells exposed to hypoxia. METHODS: The cytoprotective effects of HB-EGF were analyzed by placing IEC-18 cells in an anaerobic chamber with various timed HB-EGF treatments (prehypoxia, posthypoxia, pre- and posthypoxia, and no treatment). After 10 hours of hypoxia, lactate dehydrogenase (LDH) release, actin-filament (structural) integrity, adenosine triphosphate ( ATP) levels, and posthypoxia proliferative activity were evaluated. RESULTS: LDH analysis showed that HB-EGF exerted a cytoprotective effect during hypoxia. Pretreated cells had a significantly lower death rate during recovery (7.48%) compared with cells with no HB-EGF treatment (22.19%, P < .009). Confocal microscopic structural analysis of posthypoxia cells showed that F-actin structure was maintained in treated cells, whereas nontreated cells showed increased structural deterioration. ATP levels were significantly higher in the HB-EGF-treated cells compared with nontreated cells at 48 hours (P < .05). Finally, HB-EGF-treated cells had a significantly improved proliferative ability compared with nontreated cells during recovery from hypoxia (P < .05). CONCLUSIONS:
HB-EGF is a mitogenic growth factor for intestinal epithelial cells. Moreover, HB-EGF appears to protect intestinal epithelial cells from hypoxia, in part via maintenance of cytoskeletal structure and ATP stores. Finally, HB-EGF-treated cells also appear to have better proliferative abilities during recovery from hypoxia.
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Authors | S B Pillai, M A Turman, G E Besner |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 33
Issue 7
Pg. 973-8; discussion 978-9
(Jul 1998)
ISSN: 0022-3468 [Print] United States |
PMID | 9694080
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Actins
- Fluorescent Dyes
- Mitogens
- Epidermal Growth Factor
- Adenosine Triphosphate
- L-Lactate Dehydrogenase
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Topics |
- Actins
(metabolism)
- Adenosine Triphosphate
(metabolism)
- Analysis of Variance
- Cell Death
- Cell Hypoxia
- Cell Line
- Cytoprotection
- Epidermal Growth Factor
(pharmacology)
- Epithelial Cells
(metabolism)
- Fluorescent Dyes
- Humans
- Immunohistochemistry
- Intestinal Mucosa
(cytology, metabolism)
- L-Lactate Dehydrogenase
(metabolism)
- Mitogens
(pharmacology)
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