Abstract | BACKGROUND: METHODS: FN protein synthesis by human tubular epithelial cells in culture (TEC) was measured by biosynthetic labeling and ELISA. Splicing of FN was assessed by RT-PCR and by Northern blotting. RESULTS: Cultivated TEC synthesized and released FN, the majority of which was deposited as an unsoluble protein and a minor portion (10 to 15%) was released into the supernatant. TGF-beta and, to a lesser degree, PDGF, up-regulated FN synthesis. All three FN splice variants (EDA, EDB, and IIICS) were produced. PDGF did not influence the splicing. TGF-beta preferentially up-regulated the EDA splice variant, but had no effect on the splicing of the other domains. CONCLUSIONS: PDGF and TGF-beta both up-regulate FN synthesis of TEC. TGF-beta, but not PDGF, also changed the quality of the de novo synthesized FN, and thus has a different role in the development of sclerosis.
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Authors | A Bürger, C Wagner, C Viedt, B Reis, F Hug, G M Hänsch |
Journal | Kidney international
(Kidney Int)
Vol. 54
Issue 2
Pg. 407-15
(Aug 1998)
ISSN: 0085-2538 [Print] United States |
PMID | 9690207
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fibronectins
- Platelet-Derived Growth Factor
- RNA, Messenger
- Transforming Growth Factor beta
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Topics |
- Cells, Cultured
- Epithelial Cells
(metabolism)
- Fibronectins
(biosynthesis, genetics)
- Humans
- Kidney Tubules
(cytology, metabolism)
- Platelet-Derived Growth Factor
(pharmacology)
- RNA Splicing
- RNA, Messenger
(analysis)
- Transforming Growth Factor beta
(pharmacology)
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