Fibroblast growth factor receptor expression reflects cellular differentiation in human oral squamous carcinoma cell lines.

Abstract

This study examined the expression of fibroblast growth factor receptor 2 (FGFR 2) splice variants, IIIb and IIIc, in normal and malignant human oral keratinocytes and in normal oral fibroblasts by RT-PCR using both exon-specific primers and primers common to both FGFR 2 isoforms. Fibroblasts expressed exclusively FGFR 2/IIIc whilst the normal and malignant keratinocytes co-expressed FGFR 2/IIIb and FGFR 2/IIIc. Well-differentiated keratinocytes expressed proportionally more FGFR 2/IIIb than IIIc whereas the poorly-differentiated cells expressed more FGFR 2/IIIc than IIIb. The normal and malignant keratinocytes, but not fibroblasts, expressed an additional amplification product, which consisted of both IIIb and IIIc of FGFR 2 joined by an extra base pair and with the intronic sequence removed. The results indicate that the expression of FGFR 2 isoforms reflects the degree of cellular differentiation in normal and malignant human oral keratinocytes and that receptor complexes of FGFR 2/IIIb and IIIc may regulate ligand-receptor interactions.

• Authors: C S Drugan, I C Paterson, S S Prime
• Journal: Carcinogenesis (Carcinogenesis) Vol. 19 Issue 6 Pg. 1153-6 (Jun 1998) ISSN: 0143-3334 [Print] England
• PMID: 9667757 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Fibroblast Growth Factor
• FGFR2 protein, human
• Receptor Protein-Tyrosine Kinases
• Receptor, Fibroblast Growth Factor, Type 2

Topics

• Carcinoma, Squamous Cell (genetics, pathology)
• Cell Differentiation (genetics)
• Exons
• Humans
• Mouth Neoplasms (genetics, pathology)
• Polymerase Chain Reaction
• RNA Splicing
• Receptor Protein-Tyrosine Kinases (genetics)
• Receptor, Fibroblast Growth Factor, Type 2
• Receptors, Fibroblast Growth Factor (genetics)