Abstract |
Interleukin-12 (IL-12), a key cytokine in immune regulation, has an important role in activating the cell-mediated immune response in infectious diseases. Recently, a dichotomy between IL-12 and IL-10 regarding progression of a variety diseases has emerged. IL-12 activates type 1 cytokine production and has an antagonistic effect on type 2 cytokines. Here, by using quantitative competitive PCR, we show that peripheral blood mononuclear cells from bovine leukemia virus-infected animals in the alymphocytotic stage of disease express an increased amount of IL-12 p40 mRNA. In contrast, IL-12 p40 mRNA expression by cells from animals with late-stage disease, termed persistent lymphocytosis, was significantly decreased compared to that by normal and alymphocytotic animals. Interestingly, IL-12 p40 mRNA was also detected in tumor-bearing animals. IL-12 p40 expression occurred only in monocytes/macrophages, not B or T lymphocytes. The present study combined with previous findings suggest that IL-12 in bovine leukemia virus-infected animals may regulate production of other cytokines such as gamma interferon and IL-10 and the progression of bovine leukosis in animals that develop more advanced disease such as a persistent lymphocytosis of B cells or B-cell lymphosarcoma.
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Authors | D Pyeon, G A Splitter |
Journal | Journal of virology
(J Virol)
Vol. 72
Issue 8
Pg. 6917-21
(Aug 1998)
ISSN: 0022-538X [Print] United States |
PMID | 9658146
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- RNA, Messenger
- Interleukin-12
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Topics |
- Animals
- Cattle
- Enzootic Bovine Leukosis
(metabolism, physiopathology)
- Female
- Gene Expression
- Interleukin-12
(biosynthesis, genetics)
- Leukemia Virus, Bovine
(physiology)
- Lymphocytosis
(metabolism, physiopathology)
- Macrophages
(metabolism)
- Monocytes
(metabolism)
- RNA, Messenger
- Virus Latency
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