HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Transcript-specific effects of adrenalectomy on seizure-induced BDNF expression in rat hippocampus.

Abstract
Activity-induced brain-derived neurotrophic factor (BDNF) expression is negatively modulated by circulating adrenal steroids. The rat BDNF gene gives rise to four major transcript forms that each contain a unique 5' exon (I-IV) and a common 3' exon (V) that codes for BDNF protein. Exon-specific in situ hybridization was used to determine if adrenalectomy has differential effects on basal and activity-induced BDNF transcript expression in hippocampus. Adrenalectomy alone had only modest effects on BDNF mRNA levels with slight increases in exon III-containing mRNA with 7-10-day survival and in exon II-containing mRNA with 30-days survival. In the dentate gyrus granule cells, adrenalectomy markedly potentiated increases in exon I and II cRNA labeling, but not increases in exon III and IV cRNA labeling, elicited by one hippocampal afterdischarge. Similarly, for the granule cells and CA1 pyramidal cells, hilus lesion (HL)-induced recurrent limbic seizures elicited greater increases in exon I and II cRNA hybridization in adrenalectomized (ADX) as compared to adrenal-intact rats. In this paradigm, adrenalectomy modestly potentiated the increase in exon III-containing mRNA in CA1 but had no effect on exon IV-containing mRNA content. These results demonstrate that the negative effects of adrenal hormones on activity-induced BDNF expression are by far the greatest for transcripts containing exons I and II. Together with evidence for region-specific transcript expression, these results suggest that the effects of stress on adaptive changes in BDNF signalling will be greatest for neurons that predominantly express transcripts I and II.
AuthorsJ C Lauterborn, F R Poulsen, C T Stinis, P J Isackson, C M Gall
JournalBrain research. Molecular brain research (Brain Res Mol Brain Res) Vol. 55 Issue 1 Pg. 81-91 (Mar 30 1998) ISSN: 0169-328X [Print] Netherlands
PMID9645963 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Adrenal Cortex Hormones
  • Brain-Derived Neurotrophic Factor
  • Nerve Tissue Proteins
  • RNA, Messenger
Topics
  • Adrenal Cortex Hormones (physiology)
  • Adrenalectomy
  • Animals
  • Brain-Derived Neurotrophic Factor (biosynthesis, genetics)
  • Dentate Gyrus (injuries, metabolism)
  • Electric Injuries (genetics, metabolism)
  • Exons (genetics)
  • Gene Expression Regulation
  • Hippocampus (metabolism)
  • Male
  • Nerve Tissue Proteins (biosynthesis, genetics)
  • Pyramidal Cells (metabolism)
  • RNA, Messenger (biosynthesis, genetics)
  • Rats
  • Rats, Sprague-Dawley
  • Seizures (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: