Cultured fucosidosis fibroblasts: a simple technique demonstrating storage of tritiated-fucose labeled material.

Abstract

Incorporation of tritiated fucose into the acid-soluble and acid-insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10-day period. Fucosidosis cells incorporated significantly more label into the acid-soluble, but not the acid-insoluble fraction, than control cells. The ratio of acid-insoluble radioactivity increased lineraly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.

• Authors: S Wood
• Journal: Clinical genetics (Clin Genet) Vol. 10 Issue 3 Pg. 183-6 (Sep 1976) ISSN: 0009-9163 [Print] Denmark
• PMID: 963907 (Publication Type: Journal Article)

Chemical References

• Tritium
• Fucose
• Disaccharidases
• alpha-L-Fucosidase

Topics

• Carbohydrate Metabolism, Inborn Errors (metabolism)
• Cells, Cultured
• Disaccharidases (deficiency)
• Fibroblasts (metabolism)
• Fucose (metabolism)
• Humans
• Tritium
• alpha-L-Fucosidase (deficiency)