Suppression of acute and protracted-relapsing experimental allergic encephalomyelitis by nasal administration of low-dose IL-10 in rats.

Abstract

In this study we report for the first time that nasal administration of the Th2 cell-related cytokine interleukin-10 (IL-10), at concentrations of 1.5 microg/rat and 15 microg/rat, suppressed clinical signs of acute experimental allergic encephalomyelitis (EAE) in Lewis rats and prevented the development and relapse of protracted-relapsing EAE (PR-EAE) in DA rats. In contrast, subcutaneous injection of IL-10 (15 microg/rat) did not inhibit acute EAE. The IL-10-mediated suppression of EAE was associated with decreased myelin antigen-specific T-cell proliferative responses and IFN-gamma secretion in both acute and PR-EAE. In sections of spinal cords derived from rats nasally pretreated with IL-10, there were no infiltrating CD4+ T cells or macrophages, which are considered as major encephalitogenic or inflammatory cells. Most interestingly, nasally administered IL-10 also inhibited MHC class II expression in microglia, indicating that IL-10 administration by the nasal route prevents the activation of microglia. Administration of cytokines via the nasal route offers an exciting alternative in the prevention and treatment of autoimmune diseases.

• Authors: B G Xiao, X F Bai, G X Zhang, H Link
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 84 Issue 2 Pg. 230-7 (Apr 15 1998) ISSN: 0165-5728 [Print] Netherlands
• PMID: 9628468 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Histocompatibility Antigens Class II
• Myelin Basic Protein
• Interleukin-10
• Interferon-gamma

Topics

• Acute Disease
• Administration, Intranasal
• Animals
• Biomarkers
• CD4-Positive T-Lymphocytes (immunology)
• Dose-Response Relationship, Drug
• Encephalomyelitis, Autoimmune, Experimental (immunology)
• Guinea Pigs
• Histocompatibility Antigens Class II (metabolism)
• Immunohistochemistry
• Immunotherapy (methods)
• Interferon-gamma (metabolism)
• Interleukin-10 (administration & dosage)
• Lymphocyte Activation (drug effects, immunology)
• Macrophages (immunology)
• Microglia (immunology, metabolism)
• Myelin Basic Protein (pharmacology)
• Rats
• Rats, Inbred Lew
• Recurrence
• Spinal Cord (cytology, immunology)