Abstract |
The importance of antibodies for elimination of polyomavirus infection and the prevention of virus induced oncogenesis was studied, X-linked immunodeficiency (XID) mice, IgM-/- single knockout and IgMI-/- CD8-/- double knockout mice, all defective in antibody production, and normal control mice were infected with polyomavirus as adults. The mice were followed for presence of polyoma DNA with a polyoma specific polymerase chain reaction (PCR) over 6 weeks post infection (p.i.), a time point at which polyomavirus DNA is no longer detected in normal adult infected mice. As expected, virus DNA was not detected in normal mice 6 weeks p.i. In both IgM-/- single knockout and IgM-/- CD8-/- double knockout mice a disseminated infection was still observed by 6 weeks p.i. and the latter group of mice succumbed around two months p.i. In XID mice, only one third of the mice were still positive for viral DNA 6 weeks p.i. No polyomavirus induced tumors were observed in any of the mice during the 2-4 month observation period.
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Authors | Z Berke, H Mellin, S Heidari, T Wen, A Berglöf, G Klein, T Dalianis |
Journal | In vivo (Athens, Greece)
(In Vivo)
1998 Mar-Apr
Vol. 12
Issue 2
Pg. 143-8
ISSN: 0258-851X [Print] Greece |
PMID | 9627794
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- CD8 Antigens
- DNA, Viral
- Immunoglobulin M
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Topics |
- Animals
- Antibodies, Viral
(blood)
- CD8 Antigens
(genetics)
- DNA, Viral
(analysis)
- Female
- Genetic Linkage
- Hemagglutination Inhibition Tests
- Immunoglobulin M
(deficiency, genetics, physiology)
- Immunologic Deficiency Syndromes
(genetics, immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Inbred CBA
- Mice, Knockout
- Polyomavirus
(genetics, immunology)
- Polyomavirus Infections
(genetics, immunology)
- Tumor Virus Infections
(genetics, immunology)
- X Chromosome
(immunology)
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