Effect of herbimycin A on hsp30 and hsp70 heat shock protein gene expression in Xenopus cultured cells.

Abstract	We have examined the effect of herbimycin A, a benzoquinoid ansamycin antibiotic, on the pattern of gene expression in amphibians. Exposure of Xenopus laevis A6 kidney epithelial cells to 1 microgram/mL herbimycin A induced the synthesis of the heat shock proteins hsp30 and hsp70 as well as 33- and 45-kDa proteins. Enhanced synthesis of a 34-kDa protein appears to be specific to herbimycin A because its synthesis did not increase after heat shock (35 degrees C). In addition, the synthesis of hsp30 and hsp70 induced by herbimycin A was accompanied by an increase in their mRNAs. Herbimycin A induced a transient accumulation of hsp30 and hsp70 mRNA, which peaked between 4 and 6 h. Finally, concurrent treatment of cells with 0.5 microgram/mL herbimycin A and a mild heat shock of 27 degrees C yielded a synergistic accumulation of hsp30 and hsp70 mRNA. These studies demonstrate that herbimycin A can induce the expression of a set of stress proteins in amphibians and that concurrent treatment with herbimycin A and mild heat shock has a synergistic effect on the accumulation of hsp30 and hsp70 mRNA.
Authors	D Briant, N Ohan, J J Heikkila
Journal	Biochemistry and cell biology = Biochimie et biologie cellulaire (Biochem Cell Biol) Vol. 75 Issue 6 Pg. 777-82 ( 1997) ISSN: 0829-8211 [Print] Canada
PMID	9599667 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Benzoquinones HSP30 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins Herbicides Lactams, Macrocyclic Membrane Proteins Quinones RNA, Messenger Xenopus Proteins hsp30 protein, Xenopus Rifabutin herbimycin Protein-Tyrosine Kinases
Topics	Animals Benzoquinones Cell Line Epithelial Cells Gene Expression Regulation (drug effects) HSP30 Heat-Shock Proteins HSP70 Heat-Shock Proteins (biosynthesis, drug effects, genetics) Heat-Shock Proteins (biosynthesis, drug effects, genetics) Herbicides (pharmacology) Hot Temperature Kidney (cytology) Lactams, Macrocyclic Membrane Proteins (biosynthesis, drug effects, genetics) Protein-Tyrosine Kinases (antagonists & inhibitors) Quinones (pharmacology) RNA, Messenger (drug effects, metabolism) Rifabutin (analogs & derivatives) Xenopus Proteins Xenopus laevis

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