Abstract | OBJECTIVE: DESIGN: Functional APC resistance testing was performed using both the first-generation COATEST APC Resistance Kit and a modified, or second-generation, version of the COATEST assay that uses predilution of the patient sample with factor V-deficient plasma. Factor V genotyping for APC resistance (FV R506Q) was performed using a well-characterized polymerase chain reaction-restriction fragment length polymorphism method. SETTING: University medical center. PATIENTS: MAIN OUTCOME MEASURE: Sensitivity and specificity as determined by comparing functional APC resistance to the FV R506Q genotype. RESULTS: In 73 patients referred for hypercoagulability testing, but not receiving warfarin therapy, a sensitivity of 0.86 and a specificity of 0.75 were obtained with the first-generation COATEST assay. In contrast, a sensitivity and specificity of 1.0 were obtained when the second-generation COATEST assay was employed. In 29 patients receiving warfarin, the first-generation assay exhibited a sensitivity and specificity of 0.88 and 0.95, respectively, whereas the sensitivity and specificity for the second-generation assay was 1.0. CONCLUSIONS: Predilution of patient plasma with factor V-deficient plasma results in improved sensitivity and specificity of the COATEST APC Resistance Kit, thus offering a simple modification to enhance APC resistance determination in the routine clinical laboratory setting.
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Authors | F J Strobl, S Hoffman, S Huber, E C Williams, K V Voelkerding |
Journal | Archives of pathology & laboratory medicine
(Arch Pathol Lab Med)
Vol. 122
Issue 5
Pg. 430-3
(May 1998)
ISSN: 0003-9985 [Print] United States |
PMID | 9593344
(Publication Type: Journal Article)
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Chemical References |
- Protein C
- Reagent Kits, Diagnostic
- Warfarin
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Topics |
- Blood Coagulation Disorders
(diagnosis)
- Blood Coagulation Tests
(methods)
- Evaluation Studies as Topic
- Factor V Deficiency
(blood, drug therapy)
- Humans
- Partial Thromboplastin Time
- Protein C
(pharmacology)
- Reagent Kits, Diagnostic
- Sensitivity and Specificity
- Warfarin
(therapeutic use)
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