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Fas-mediated cytotoxicity induces degradation of vesicular stomatitis virus RNA transcripts and reduces viral titer.

Abstract
Several investigators have recently examined the effect of Fas (CD95)-mediated apoptotic cell death on target cells (TC). The effect of Fas-mediated death on viral RNA within the TC, however, has not been explored. In this study, we investigated the ability of the Fas pathway to mediate pre-lytic degradation of vesicular stomatitis virus (VSV) RNA and TC RNA. We show that engagement of Fas antigen on VSV-infected Jurkat cells induces pre-lytic degradation of VSV RNA transcripts, whereas full-length VSV genome RNA, known to be tightly associated with viral proteins, is not degraded. Cellular RNA, including beta-actin and glyceraldehyde-3-phosphate-dehydrogenase mRNAs, is also degraded by Fas-mediated cytotoxicity. In addition, Fas-mediated cytotoxicity reduced the yield of VSV plaque-forming units (PFU) from Jurkat by an average of 82.0%. An anti-Fas blocking Ab inhibited the RNA degradation and restored the number of VSV PFU to near control levels. These data indicate that the Fas lytic pathway could play a role in the elimination of viruses through degradation of intracellular viral RNA. reserved
AuthorsA H Montel, G Hommel-Berrey, Z Brahmi
JournalMolecular immunology (Mol Immunol) Vol. 34 Issue 15 Pg. 1055-66 (Oct 1997) ISSN: 0161-5890 [Print] ENGLAND
PMID9519764 (Publication Type: Journal Article)
Chemical References
  • Actins
  • Antigens, CD95
  • RNA, Messenger
  • RNA, Viral
  • Glyceraldehyde-3-Phosphate Dehydrogenases
Topics
  • Actins (genetics)
  • Animals
  • Antigens, CD95 (immunology)
  • Cricetinae
  • Cytotoxicity, Immunologic
  • Glyceraldehyde-3-Phosphate Dehydrogenases (genetics)
  • Humans
  • Jurkat Cells
  • RNA, Messenger (metabolism)
  • RNA, Viral (metabolism)
  • Transcription, Genetic
  • Vesicular stomatitis Indiana virus (genetics)
  • Viral Load

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