Significance of platelet-derived endothelial cell growth factor in the angiogenesis of human gastric cancer.

Abstract

We have previously shown that platelet-derived endothelial cell growth factor (PD-ECGF) is associated with angiogenesis of human colon cancer; this factor is expressed at high levels in vascular tumors that express low levels of vascular endothelial growth factor (VEGF). In these colon cancers, the major source of PD-ECGF is the infiltrating cells. In this study, we examined the role of PD-ECGF in the angiogenesis of human gastric cancer. Immunostaining for PD-ECGF was done on 93 gastric cancers previously stained for VEGF, basic fibroblast growth factor, and factor VIII-related antigen (specific for endothelial cells). To determine the cell type expressing PD-ECGF, double staining was done using antibodies to both PD-ECGF and CD68 (specific for macrophages). PD-ECGF was expressed more frequently in infiltrating cells (positive CD68 staining; 53.8%) than in tumor epithelium (9.7%; P < 0.0001). Infiltrating cells simultaneously stained positive for both PD-ECGF and CD68. An association between PD-ECGF expression in infiltrating cells, VEGF expression in tumor epithelium, and vessel count was observed in intestinal-type gastric cancer but not in diffuse-type gastric cancer. Vessel count was greater in tumors with high expression of both PD-ECGF and VEGF than in those with high expression of either factor alone (P = 0.002). Multiple angiogenic factors expressed by both tumor cells and infiltrating cells may play a role in the regulation of angiogenesis in intestinal-type gastric cancer.

• Authors: Y Takahashi, C D Bucana, Y Akagi, W Liu, K R Cleary, M Mai, L M Ellis
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 4 Issue 2 Pg. 429-34 (Feb 1998) ISSN: 1078-0432 [Print] United States
• PMID: 9516932 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Endothelial Growth Factors
• Lymphokines
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Thymidine Phosphorylase

Topics

• Adult
• Aged
• Endothelial Growth Factors (biosynthesis)
• Female
• Humans
• Immunohistochemistry
• Lymphokines (biosynthesis)
• Male
• Middle Aged
• Neoplasm Staging
• Neovascularization, Pathologic (metabolism)
• Stomach Neoplasms (blood supply, pathology)
• Thymidine Phosphorylase (biosynthesis)
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors