Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells.

Abstract	We investigated the expression of protein disulfide isomerase family proteins (PDI, ERp61, and ERp72) in mouse F9 teratocarcinoma cells during differentiation induced by treatment with retinoic acid and dibutyryl cAMP. Each member of this family was expressed at a constitutive level in undifferentiated F9 cells. During differentiation of F9 cells to parietal or visceral endodermal cells the protein level of all these enzymes increased, although the extent of this increase in both protein and mRNA levels varied among the enzymes. Certain proteins were found to be coimmunoprecipitated with PDI, ERp61, and ERp72 in the presence of a chemical crosslinker. Type IV collagen was significantly coprecipitated with PDI whereas laminin was equally coprecipitated with the three proteins. Furthermore, 210 kDa protein characteristically coprecipitated with ERp72. Thus, the induction of PDI family proteins during the differentiation of F9 cells and their association with different proteins may implicate specific functions of each member of this family despite the common redox activity capable of catalyzing the disulfide bond formation.
Authors	O Miyaishi, K Kozaki, K Iida, K Isobe, Y Hashizume, S Saga
Journal	Journal of cellular biochemistry (J Cell Biochem) Vol. 68 Issue 4 Pg. 436-45 (Mar 15 1998) ISSN: 0730-2312 [Print] United States
PMID	9493907 (Publication Type: Journal Article)
Chemical References	Heat-Shock Proteins Membrane Glycoproteins endoplasmic reticulum glycoprotein p72 Tretinoin Bucladesine Isomerases Pdia3 protein, mouse Protein Disulfide-Isomerases PDIA3 protein, human
Topics	Animals Blotting, Northern Blotting, Western Bucladesine (pharmacology) Cell Differentiation (physiology) Electrophoresis, Polyacrylamide Gel Endoderm (cytology, enzymology) Enzyme Induction Fluorescent Antibody Technique Heat-Shock Proteins (biosynthesis) Humans Isomerases (biosynthesis) Membrane Glycoproteins (biosynthesis) Mice Precipitin Tests Protein Disulfide-Isomerases (biosynthesis) Stem Cells (cytology, enzymology) Teratocarcinoma Tretinoin (pharmacology) Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!