Abstract | AIMS: METHODS:
DNA and RNA were isolated simultaneously from formalin fixed paraffin wax embedded tissue blocks and subjected to the polymerase chain reaction (PCR) and semi-nested reverse transcription PCR (RT-PCR), respectively. The RT-PCR T cell receptor V beta products were analysed by CDR3 spectratyping using a denaturing polyacrylamide gel and silver staining. RESULTS: Usable DNA and RNA were isolated simultaneously from formalin fixed paraffin wax embedded tissue. The specific clonality of the tissue was successfully analysed by a non-radioactive method of T cell receptor CDR3 spectratyping of the RT-PCR products. CDR3 spectratying of the RT-PCR products demonstrated the precise clonal nature of the tumour and non-tumour tissue showing that the non-tumour tissue comprised an oligoclonal population of a number of different T cell receptor V beta families. The tumour tissue comprised two T cell subtypes of the one family, T cell receptor V beta 9. CONCLUSIONS:
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Authors | U O'Shea, J I Wyatt, P D Howdle |
Journal | Journal of clinical pathology
(J Clin Pathol)
Vol. 50
Issue 10
Pg. 811-4
(Oct 1997)
ISSN: 0021-9746 [Print] England |
PMID | 9462260
(Publication Type: Journal Article)
|
Chemical References |
- DNA, Neoplasm
- RNA, Neoplasm
- Receptor-CD3 Complex, Antigen, T-Cell
- Formaldehyde
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Topics |
- DNA, Neoplasm
(analysis)
- Electrophoresis, Polyacrylamide Gel
- Formaldehyde
- Humans
- Lymphoma, T-Cell
(genetics)
- Paraffin Embedding
- Polymerase Chain Reaction
(methods)
- RNA, Neoplasm
(analysis)
- Receptor-CD3 Complex, Antigen, T-Cell
(analysis)
- Silver Staining
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