Induction of TSC-22 by treatment with a new anti-cancer drug, vesnarinone, in a human salivary gland cancer cell.

Abstract

We undertook the present study to clarify the molecular mechanism of the effect of a new anti-cancer drug, vesnarinone, on a human salivary gland cancer cell line, TYS. We isolated TSC-22cDNA as avesnarinone-inducible gene from a cDNA library constructed from vesnarinone-treated TYS cells. TSC-22 was originally reported as a transforming growth factor (TGF)-beta-inducible gene. The expression of TSC-22 was up-regulated within a few hours after treatment with vesnarinone and was continued for 3 days. The level of TSC-22 mRNA in TYS cells was continuously increased until the cells reached confluency. Furthermore, the induction of TSC-22 by vesnarinone was inhibited by treatment with cycloheximide. When we treated the cells with an antisense oligonucleotide against TSC-22 mRNA under quiescent conditions, the antisense oligonucleotide stimulated the growth of TYS cells; however, under growing conditions the antisense oligonucleotide did not affect cell growth. Furthermore, the antisense oligonucleotide suppressed the antiproliferative effect of vesnarinone. These results suggest that TSC-22 may be a negative growth regulator and may play an important role in the antiproliferative effect of vesnarinone.

• Authors: H Kawamata, K Nakashiro, D Uchida, S Hino, F Omotehara, H Yoshida, M Sato
• Journal: British journal of cancer (Br J Cancer) Vol. 77 Issue 1 Pg. 71-8 ( 1998) ISSN: 0007-0920 [Print] England
• PMID: 9459148 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Neoplasm Proteins
• Oligonucleotides, Antisense
• Pyrazines
• Quinolines
• RNA, Messenger
• Repressor Proteins
• TSC22D1 protein, human
• Transcription Factors
• vesnarinone

Topics

• Antineoplastic Agents (pharmacology)
• Cell Cycle (drug effects)
• Cell Division (drug effects)
• Humans
• Neoplasm Proteins (biosynthesis, genetics)
• Oligonucleotides, Antisense (pharmacology)
• Pyrazines
• Quinolines (pharmacology)
• RNA, Messenger (biosynthesis, drug effects)
• Repressor Proteins
• Salivary Gland Neoplasms (genetics, metabolism, pathology)
• Transcription Factors (biosynthesis, genetics)
• Tumor Cells, Cultured (drug effects)
• Up-Regulation