Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose.

Abstract

A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbent is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatographic procedure is simply adsorption, wash, elution. The procedure is simple, efficient, and inexpensive.

• Authors: T C Bøg-Hansen
• Journal: Molecular biotechnology (Mol Biotechnol) Vol. 8 Issue 3 Pg. 279-81 (Dec 1997) ISSN: 1073-6085 [Print] United States
• PMID: 9438262 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Culture Media
• Sepharose

Topics

• Antibodies, Monoclonal (isolation & purification)
• Ascitic Fluid
• Cells, Cultured
• Culture Media
• Sepharose