Abstract | OBJECTIVE: The definition of plasmacytoid myoepithelioma, a neoplasm exhibiting myoepithelial differentiation, has been recently questioned. To better understand the histogenesis of this neoplasm, we searched for myoepithelial markers in histologic sections of plasmacytoid myoepithelioma and in a cell line (M1) derived from this same neoplasm. STUDY DESIGN: Expression of vimentin, pan- keratin (AE-3) and smooth-muscle actin was studied by immunohistochemistry in paraffin-embedded tissue and by immunofluorescence in M1 cells. RESULTS: Plasmacytoid myoepithelioma tumor sections showed vimentin and AE-3 reactivity, but evidence of smooth-muscle actin was not seen. The cell line derived from this tumor also produced vimentin and cytokeratin. In addition, all cultured cells expressed smooth-muscle actin. CONCLUSION: We demonstrated that cells derived from a case of plasmacytoid myoepithelioma appear to show full myoepithelial differentiation in vitro. Thus, they are myoepithelial-like cells in nature. The lack of myogenous differentiation in vivo could be due to an inhibitory process mediated by the extracellular matrix.
|
Authors | R G Jaeger, P T de Oliveira, M M Jaeger, V C de Araújo |
Journal | Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
(Oral Surg Oral Med Oral Pathol Oral Radiol Endod)
Vol. 84
Issue 6
Pg. 663-7
(Dec 1997)
ISSN: 1079-2104 [Print] United States |
PMID | 9431536
(Publication Type: Case Reports, Journal Article)
|
Chemical References |
- Actins
- Antibodies, Monoclonal
- Biomarkers, Tumor
- Chromogenic Compounds
- Coloring Agents
- Vimentin
- 3,3'-Diaminobenzidine
- Keratins
- Fluorescein
|
Topics |
- 3,3'-Diaminobenzidine
- Actins
(analysis, genetics)
- Antibodies, Monoclonal
- Biomarkers, Tumor
(analysis)
- Cell Differentiation
- Chromogenic Compounds
- Coloring Agents
- Extracellular Matrix
(physiology)
- Female
- Fluorescein
- Fluorescent Antibody Technique, Direct
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Keratins
(analysis, genetics)
- Microscopy, Fluorescence
- Middle Aged
- Myoepithelioma
(pathology)
- Palatal Neoplasms
(pathology)
- Paraffin Embedding
- Plasma Cells
(pathology)
- Tumor Cells, Cultured
- Vimentin
(analysis, genetics)
|