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Expression of smooth-muscle actin in cultured cells from human plasmacytoid myoepithelioma.

AbstractOBJECTIVE:
The definition of plasmacytoid myoepithelioma, a neoplasm exhibiting myoepithelial differentiation, has been recently questioned. To better understand the histogenesis of this neoplasm, we searched for myoepithelial markers in histologic sections of plasmacytoid myoepithelioma and in a cell line (M1) derived from this same neoplasm.
STUDY DESIGN:
Expression of vimentin, pan-keratin (AE-3) and smooth-muscle actin was studied by immunohistochemistry in paraffin-embedded tissue and by immunofluorescence in M1 cells.
RESULTS:
Plasmacytoid myoepithelioma tumor sections showed vimentin and AE-3 reactivity, but evidence of smooth-muscle actin was not seen. The cell line derived from this tumor also produced vimentin and cytokeratin. In addition, all cultured cells expressed smooth-muscle actin.
CONCLUSION:
We demonstrated that cells derived from a case of plasmacytoid myoepithelioma appear to show full myoepithelial differentiation in vitro. Thus, they are myoepithelial-like cells in nature. The lack of myogenous differentiation in vivo could be due to an inhibitory process mediated by the extracellular matrix.
AuthorsR G Jaeger, P T de Oliveira, M M Jaeger, V C de Araújo
JournalOral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 84 Issue 6 Pg. 663-7 (Dec 1997) ISSN: 1079-2104 [Print] United States
PMID9431536 (Publication Type: Case Reports, Journal Article)
Chemical References
  • Actins
  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • Chromogenic Compounds
  • Coloring Agents
  • Vimentin
  • 3,3'-Diaminobenzidine
  • Keratins
  • Fluorescein
Topics
  • 3,3'-Diaminobenzidine
  • Actins (analysis, genetics)
  • Antibodies, Monoclonal
  • Biomarkers, Tumor (analysis)
  • Cell Differentiation
  • Chromogenic Compounds
  • Coloring Agents
  • Extracellular Matrix (physiology)
  • Female
  • Fluorescein
  • Fluorescent Antibody Technique, Direct
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Keratins (analysis, genetics)
  • Microscopy, Fluorescence
  • Middle Aged
  • Myoepithelioma (pathology)
  • Palatal Neoplasms (pathology)
  • Paraffin Embedding
  • Plasma Cells (pathology)
  • Tumor Cells, Cultured
  • Vimentin (analysis, genetics)

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