Abstract | PURPOSE: METHODS: RESULTS:
P-glycoprotein expression was detected in 10 of 10 patients pre-exposed to intravitreal daunomycin. In contrast, epiretinal membranes from only 2 of 13 patients never exposed to daunomycin showed faint P-glycoprotein expression. P-glycoprotein expression was strong within 8 months after daunomycin treatment and faded thereafter. Colocalization studies demonstrated predominant expression of P-glycoprotein by RPE cells. Pre-exposure of cultured human RPE cells to subtoxic concentrations of daunomycin induced resistance to daunomycin that was sensitive to the MDR inhibitor, verapamil. Induction of the MDR phenotype in RPE cells by daunomycin was associated with a minor increase in the mdr-1 mRNA level but a prominent increase in P-glycoprotein expression, thus suggesting a primarily translational mechanism of MDR development in human RPE cells. CONCLUSIONS:
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Authors | P Esser, D Tervooren, K Heimann, N Kociok, K U Bartz-Schmidt, P Walter, M Weller |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 39
Issue 1
Pg. 164-70
(Jan 1998)
ISSN: 0146-0404 [Print] United States |
PMID | 9430558
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Antibiotics, Antineoplastic
- Glial Fibrillary Acidic Protein
- RNA, Messenger
- Transcription Factors
- Keratins
- Daunorubicin
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Topics |
- ATP Binding Cassette Transporter, Subfamily B, Member 1
(genetics, metabolism)
- Adolescent
- Adult
- Aged
- Antibiotics, Antineoplastic
(pharmacology, therapeutic use)
- Cell Culture Techniques
- Chemotherapy, Adjuvant
- Child, Preschool
- Daunorubicin
(pharmacology, therapeutic use)
- Drug Resistance, Multiple
- Female
- Glial Fibrillary Acidic Protein
(metabolism)
- Humans
- Immunoenzyme Techniques
- Keratins
(metabolism)
- Male
- Middle Aged
- Pigment Epithelium of Eye
(cytology, drug effects, metabolism)
- Polymerase Chain Reaction
- RNA, Messenger
(metabolism)
- Transcription Factors
(metabolism)
- Vitreoretinopathy, Proliferative
(metabolism, pathology, prevention & control)
- Vitreous Body
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