Abstract | BACKGROUND: METHODS: Rats (n = 48) were anesthetized with isoflurane and subarachnoid hemorrhage was induced by injecting 0.3 ml of autologous blood into the cistema magna. Each animal received one of the following regimens: Control, no hematocrit manipulation; DCLHb, hematocrit concentration decreased to 30% with DCLHb; or Alb, hematocrit concentration decreased to 30% with human serum albumin. The experiments had two parts, A and B. In part A, after 20 min, cerebral blood flow (CBF) was assessed with 14C-iodoantipyrine autoradiography. In part B, after 96 h, in separate animals, the number of dead neurons was determined in predetermined coronal sections by hematoxylin and eosin staining. RESULTS: Cerebral blood flow was greater for the DCLHb group than for the control group; and CBF was greater for the Alb group than the other two groups (P < 0.05). In one section, CBF was 45.5 +/- 10.9 ml x 100 g(-1) x min(-1) (mean +/- SD) for the control group, 95.3 +/- 16.6 ml x 100 g(-1) x min(-1) for the DCLHb group, and 138.1 +/- 18.7 ml x 100 g(-1) x min(-1) for the Alb group. The number of dead neurons was less in the Alb group (611 +/- 84) than in the control group (1,097 +/- 211), and was less in the DCLHb group (305 +/- 38) than in the other two groups (P < 0.05). CONCLUSIONS:
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Authors | D J Cole, J C Nary, L W Reynolds, P M Patel, J C Drummond |
Journal | Anesthesiology
(Anesthesiology)
Vol. 87
Issue 6
Pg. 1486-93
(Dec 1997)
ISSN: 0003-3022 [Print] United States |
PMID | 9416734
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hemoglobins
- diaspirin-cross-linked hemoglobin
- Aspirin
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Topics |
- Animals
- Aspirin
(analogs & derivatives, pharmacology, therapeutic use)
- Brain
(blood supply, drug effects, pathology)
- Cell Death
(drug effects)
- Cerebrovascular Circulation
(drug effects)
- Exchange Transfusion, Whole Blood
- Hemodilution
- Hemoglobins
(pharmacology, therapeutic use)
- Male
- Rats
- Rats, Inbred SHR
- Subarachnoid Hemorrhage
(physiopathology, therapy)
- Vasoconstriction
(drug effects)
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