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Polymerase chain reaction and reverse cross blot hybridization assay for detection of mycobacterial DNA in lupus vulgaris.

Abstract
For 5 years, an 83-year-old man had been suffering from slightly itchy erythematous plaques with clearcut margins, located on his left thigh and on his right arm; in addition, on his right auricle there was an erythematous patch with yellowish shadings that had appeared about 3 years before and had progressively spread to the temporal-zygomatic region, the chin and the mandibular arch. These lesions were strongly suggestive of lupus vulgaris; however the conventional bacteriological examinations performed on the biopsy specimen from lesional skin were negative. A diagnosis of lupus vulgaris was achieved through the detection of the 16S rRNA gene of Mycobacterium tuberculosis in a skin biopsy of the patient by means of a polymerase chain reaction followed by a reverse cross blot hybridization, a method which allows the identification of different mycobacterial species in a single hybridization procedure.
AuthorsM Sanguinetti, B Posteraro, L M Chinni, S Ruggeri, D B Camaioni, T Gobello, G Fadda
JournalDermatology (Basel, Switzerland) (Dermatology) Vol. 195 Issue 3 Pg. 293-6 ( 1997) ISSN: 1018-8665 [Print] Switzerland
PMID9407186 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Bacterial
Topics
  • Aged
  • Aged, 80 and over
  • DNA, Bacterial (isolation & purification)
  • Humans
  • Lupus Vulgaris (diagnosis)
  • Male
  • Mycobacterium tuberculosis (genetics)
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction

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