Abstract |
For 5 years, an 83-year-old man had been suffering from slightly itchy erythematous plaques with clearcut margins, located on his left thigh and on his right arm; in addition, on his right auricle there was an erythematous patch with yellowish shadings that had appeared about 3 years before and had progressively spread to the temporal-zygomatic region, the chin and the mandibular arch. These lesions were strongly suggestive of lupus vulgaris; however the conventional bacteriological examinations performed on the biopsy specimen from lesional skin were negative. A diagnosis of lupus vulgaris was achieved through the detection of the 16S rRNA gene of Mycobacterium tuberculosis in a skin biopsy of the patient by means of a polymerase chain reaction followed by a reverse cross blot hybridization, a method which allows the identification of different mycobacterial species in a single hybridization procedure.
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Authors | M Sanguinetti, B Posteraro, L M Chinni, S Ruggeri, D B Camaioni, T Gobello, G Fadda |
Journal | Dermatology (Basel, Switzerland)
(Dermatology)
Vol. 195
Issue 3
Pg. 293-6
( 1997)
ISSN: 1018-8665 [Print] Switzerland |
PMID | 9407186
(Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Aged
- Aged, 80 and over
- DNA, Bacterial
(isolation & purification)
- Humans
- Lupus Vulgaris
(diagnosis)
- Male
- Mycobacterium tuberculosis
(genetics)
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
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