Anti-microbial activity of human CAP18 peptides.

Abstract

BACKGROUND:

CAP18 derived from rabbit leukocytes is a 142-amino acid protein recently demonstrated to have Lipopolysaccharide (LPS) binding and anti-microbial activity. The C-terminal 37 amino acids of rabbit CAP18 (CAP18(106-142) comprise the LPS-binding and anti-microbial domain. The homologous domain of human CAP18 (huCAP18(104-140) was identified from the recently cloned human CAP18 cDNA.

OBJECTIVES:

To evaluate the antimicrobial activity of C-terminal peptides derived from human CAP18.

STUDY DESIGN:

Prepare synthetic human CAP18(104-140) and study anti-microbial activity versus various gram-negative and gram-positive bacteria.

RESULTS:

Synthetic human CAP18(104-140) has broad anti-microbial activity versus both gram-positive (IC50 = 2.5 micrograms/ml) and gram-negative bacteria (IC50 = 0.5-5 micrograms/ml). Susceptible strains include Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium. A 32-amino acid peptide lacking five amino acids from the C-terminus of CAP18(104-140) has higher activity. Unlike previously characterized anti-microbial peptides derived from granulocyte proteins, CAP18(104-140) is active in serum.

CONCLUSIONS:

Human CAP18(104-140) or a derivative peptide may have therapeutic potential for bacterial sepsis.

• Authors: J W Larrick, M Hirata, J Zhong, S C Wright
• Journal: Immunotechnology : an international journal of immunological engineering (Immunotechnology) Vol. 1 Issue 1 Pg. 65-72 (May 1995) ISSN: 1380-2933 [Print] Netherlands
• PMID: 9373334 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anti-Bacterial Agents
• Antimicrobial Cationic Peptides
• Carrier Proteins
• Cathelicidins
• Lipopolysaccharides
• Protein Sorting Signals

Topics

• Amino Acid Sequence
• Animals
• Anti-Bacterial Agents (pharmacology)
• Antimicrobial Cationic Peptides
• Carrier Proteins (pharmacology)
• Cathelicidins
• Cloning, Molecular
• Humans
• Lipopolysaccharides (metabolism, pharmacology)
• Microbial Sensitivity Tests
• Molecular Sequence Data
• Protein Sorting Signals (chemistry)
• Protein Structure, Secondary
• Rabbits